Ablation of murine jejunal crypts by alkylating agents.

The gut microcolony assay has been used to measure damage to intestinal crypts by single and split doses of 3 alkylating agents: mechlorethamine hydrochloride (HN2), bis-chloroethyl-nitrosourea (BCNU) and isopropyl methane sulphonate (IMS). The single-dose survival curves for whole crypts were disti...

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Detalles Bibliográficos
Autor principal: Moore, J. V.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1979
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009854/
https://www.ncbi.nlm.nih.gov/pubmed/219878
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author Moore, J. V.
author_facet Moore, J. V.
author_sort Moore, J. V.
collection PubMed
description The gut microcolony assay has been used to measure damage to intestinal crypts by single and split doses of 3 alkylating agents: mechlorethamine hydrochloride (HN2), bis-chloroethyl-nitrosourea (BCNU) and isopropyl methane sulphonate (IMS). The single-dose survival curves for whole crypts were distinguished by extrapolation numbers (3.0, 176 and 1.5 respectively) that were lower than most previously published values for assay by irradiation. Significant sparing of crypts occurred when doses of HN2 or BCNU, but not IMS, were given in 2 equal fractions separated by more than 2 h. Deduced D0 values for those cells from which crypts regenerate were 1.9 mg/kg HN2, 19 mg/kg BCNU and 487 mg/kg IMS.
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spelling pubmed-20098542009-09-10 Ablation of murine jejunal crypts by alkylating agents. Moore, J. V. Br J Cancer Research Article The gut microcolony assay has been used to measure damage to intestinal crypts by single and split doses of 3 alkylating agents: mechlorethamine hydrochloride (HN2), bis-chloroethyl-nitrosourea (BCNU) and isopropyl methane sulphonate (IMS). The single-dose survival curves for whole crypts were distinguished by extrapolation numbers (3.0, 176 and 1.5 respectively) that were lower than most previously published values for assay by irradiation. Significant sparing of crypts occurred when doses of HN2 or BCNU, but not IMS, were given in 2 equal fractions separated by more than 2 h. Deduced D0 values for those cells from which crypts regenerate were 1.9 mg/kg HN2, 19 mg/kg BCNU and 487 mg/kg IMS. Nature Publishing Group 1979-02 /pmc/articles/PMC2009854/ /pubmed/219878 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Moore, J. V.
Ablation of murine jejunal crypts by alkylating agents.
title Ablation of murine jejunal crypts by alkylating agents.
title_full Ablation of murine jejunal crypts by alkylating agents.
title_fullStr Ablation of murine jejunal crypts by alkylating agents.
title_full_unstemmed Ablation of murine jejunal crypts by alkylating agents.
title_short Ablation of murine jejunal crypts by alkylating agents.
title_sort ablation of murine jejunal crypts by alkylating agents.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009854/
https://www.ncbi.nlm.nih.gov/pubmed/219878
work_keys_str_mv AT moorejv ablationofmurinejejunalcryptsbyalkylatingagents

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