Purification of a protein associated with human bronchogenic squamous-cell carcinoma.

A heteroantiserum raised in rabbits to extracts of human squamous-cell carcinoma of the lung which exhibited marked tumour specificity was used to monitor the fractionation and isolation of a tumour-associated component of the extract. KC1 extracts of pools of both normal lung and bronchogenic squam...

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Detalles Bibliográficos
Autores principales: Kelly, B. S., Levy, J. G.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1979
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009874/
https://www.ncbi.nlm.nih.gov/pubmed/465295
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author Kelly, B. S.
Levy, J. G.
author_facet Kelly, B. S.
Levy, J. G.
author_sort Kelly, B. S.
collection PubMed
description A heteroantiserum raised in rabbits to extracts of human squamous-cell carcinoma of the lung which exhibited marked tumour specificity was used to monitor the fractionation and isolation of a tumour-associated component of the extract. KC1 extracts of pools of both normal lung and bronchogenic squamous-cell carcinoma were subjected to a series of purification steps involving acid precipitation, salting out, DEAE chromatography and preparative polyacrylamide-gel electrophoresis. At each stage, fractions were tested for their ability to react in the complement-fixation assay with the antiserum. A protein was ultimately isolated which did not appear to be present at detectable levels in an equivalent fraction of normal lung extract, reacted with the heteroantiserum, and appeared to be present in all extracts of squamous-cell carcinoma. IMAGES:
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spelling pubmed-20098742009-09-10 Purification of a protein associated with human bronchogenic squamous-cell carcinoma. Kelly, B. S. Levy, J. G. Br J Cancer Research Article A heteroantiserum raised in rabbits to extracts of human squamous-cell carcinoma of the lung which exhibited marked tumour specificity was used to monitor the fractionation and isolation of a tumour-associated component of the extract. KC1 extracts of pools of both normal lung and bronchogenic squamous-cell carcinoma were subjected to a series of purification steps involving acid precipitation, salting out, DEAE chromatography and preparative polyacrylamide-gel electrophoresis. At each stage, fractions were tested for their ability to react in the complement-fixation assay with the antiserum. A protein was ultimately isolated which did not appear to be present at detectable levels in an equivalent fraction of normal lung extract, reacted with the heteroantiserum, and appeared to be present in all extracts of squamous-cell carcinoma. IMAGES: Nature Publishing Group 1979-03 /pmc/articles/PMC2009874/ /pubmed/465295 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Kelly, B. S.
Levy, J. G.
Purification of a protein associated with human bronchogenic squamous-cell carcinoma.
title Purification of a protein associated with human bronchogenic squamous-cell carcinoma.
title_full Purification of a protein associated with human bronchogenic squamous-cell carcinoma.
title_fullStr Purification of a protein associated with human bronchogenic squamous-cell carcinoma.
title_full_unstemmed Purification of a protein associated with human bronchogenic squamous-cell carcinoma.
title_short Purification of a protein associated with human bronchogenic squamous-cell carcinoma.
title_sort purification of a protein associated with human bronchogenic squamous-cell carcinoma.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009874/
https://www.ncbi.nlm.nih.gov/pubmed/465295
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AT levyjg purificationofaproteinassociatedwithhumanbronchogenicsquamouscellcarcinoma

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