The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin.

The photosensitizing effect of haematoporphyrin (HP) on human cells of the established line NHIK 3025 has been studied. Fluorescence measurements show that HP is bound to these cells. Serum proteins also bind HP, and the presence of 10% human serum during incubation with HP (3 X 10(-4)M) reduces the...

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Detalles Bibliográficos
Autores principales: Moan, J., Pettersen, E. O., Christensen, T.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1979
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009925/
https://www.ncbi.nlm.nih.gov/pubmed/220998
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author Moan, J.
Pettersen, E. O.
Christensen, T.
author_facet Moan, J.
Pettersen, E. O.
Christensen, T.
author_sort Moan, J.
collection PubMed
description The photosensitizing effect of haematoporphyrin (HP) on human cells of the established line NHIK 3025 has been studied. Fluorescence measurements show that HP is bound to these cells. Serum proteins also bind HP, and the presence of 10% human serum during incubation with HP (3 X 10(-4)M) reduces the cellular uptake of HP by 75% or more. The photosensitized inactivation is enhanced when the cells are suspended in D2O-buffer during irradiation. This indicates that singlet oxygen is involved in the inactivation. Two findings indicate that the photoinduced damage is repairable: firstly, the fraction of cells surviving a given light dose decreases with decreasing irradiation temperature, and secondly, the survival curves have a shoulder at low exposures of light. IMAGES:
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spelling pubmed-20099252009-09-10 The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin. Moan, J. Pettersen, E. O. Christensen, T. Br J Cancer Research Article The photosensitizing effect of haematoporphyrin (HP) on human cells of the established line NHIK 3025 has been studied. Fluorescence measurements show that HP is bound to these cells. Serum proteins also bind HP, and the presence of 10% human serum during incubation with HP (3 X 10(-4)M) reduces the cellular uptake of HP by 75% or more. The photosensitized inactivation is enhanced when the cells are suspended in D2O-buffer during irradiation. This indicates that singlet oxygen is involved in the inactivation. Two findings indicate that the photoinduced damage is repairable: firstly, the fraction of cells surviving a given light dose decreases with decreasing irradiation temperature, and secondly, the survival curves have a shoulder at low exposures of light. IMAGES: Nature Publishing Group 1979-04 /pmc/articles/PMC2009925/ /pubmed/220998 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Moan, J.
Pettersen, E. O.
Christensen, T.
The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin.
title The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin.
title_full The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin.
title_fullStr The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin.
title_full_unstemmed The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin.
title_short The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin.
title_sort mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009925/
https://www.ncbi.nlm.nih.gov/pubmed/220998
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