A radioimmunoassay for cytosine arabinoside.

A radioimmunoassay (RIA) for cytosine arabinoside (AraC) has been developed using antiserum raised in a sheep to an AraC monophosphate-ovalbumin conjugate. The antibody shows only 0.008% cross-reactivity with uracil arabinoside (AraU) and low (0.023%) cross-reactivity with other commonly co-administ...

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Detalles Bibliográficos
Autores principales: Piall, E. M., Aherne, G. W., Marks, V. M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1979
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010074/
https://www.ncbi.nlm.nih.gov/pubmed/574019
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author Piall, E. M.
Aherne, G. W.
Marks, V. M.
author_facet Piall, E. M.
Aherne, G. W.
Marks, V. M.
author_sort Piall, E. M.
collection PubMed
description A radioimmunoassay (RIA) for cytosine arabinoside (AraC) has been developed using antiserum raised in a sheep to an AraC monophosphate-ovalbumin conjugate. The antibody shows only 0.008% cross-reactivity with uracil arabinoside (AraU) and low (0.023%) cross-reactivity with other commonly co-administered drugs such as cytotoxic and antibacterial agents, and also a number of naturally occurring nucleosides and nucleotides. It does however cross-react by 125% with AraC monophosphate and by 109% with AraC triphosphate. As little as 1 ng/ml of AraC can be detected in plasma, serum, urine and cerebrospinal fluid (CSF) with no need for prior extraction. This RIA has been used to follow the disappearance of AraC from the plasma of patients receiving the drug.
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spelling pubmed-20100742009-09-10 A radioimmunoassay for cytosine arabinoside. Piall, E. M. Aherne, G. W. Marks, V. M. Br J Cancer Research Article A radioimmunoassay (RIA) for cytosine arabinoside (AraC) has been developed using antiserum raised in a sheep to an AraC monophosphate-ovalbumin conjugate. The antibody shows only 0.008% cross-reactivity with uracil arabinoside (AraU) and low (0.023%) cross-reactivity with other commonly co-administered drugs such as cytotoxic and antibacterial agents, and also a number of naturally occurring nucleosides and nucleotides. It does however cross-react by 125% with AraC monophosphate and by 109% with AraC triphosphate. As little as 1 ng/ml of AraC can be detected in plasma, serum, urine and cerebrospinal fluid (CSF) with no need for prior extraction. This RIA has been used to follow the disappearance of AraC from the plasma of patients receiving the drug. Nature Publishing Group 1979-10 /pmc/articles/PMC2010074/ /pubmed/574019 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Piall, E. M.
Aherne, G. W.
Marks, V. M.
A radioimmunoassay for cytosine arabinoside.
title A radioimmunoassay for cytosine arabinoside.
title_full A radioimmunoassay for cytosine arabinoside.
title_fullStr A radioimmunoassay for cytosine arabinoside.
title_full_unstemmed A radioimmunoassay for cytosine arabinoside.
title_short A radioimmunoassay for cytosine arabinoside.
title_sort radioimmunoassay for cytosine arabinoside.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010074/
https://www.ncbi.nlm.nih.gov/pubmed/574019
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