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Effect of human, bovine and ovine prolactin on DNA synthesis by organ cultures of benign human breast tumours.

Ten benign breast tumours from 9 female patients (8 with fibrocystic disease and 1 with fibroadenoma) and 1 male patient (with gynaecomastia) were processed into slices and individually cultured for 2 days in serum-free Medium 199. [3H]-TdR was added to the culture medium to assess DNA synthesis. Th...

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Autores principales: Welsch, C. W., Dombroske, S. E., McManus, M. J., Calaf, G.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1979
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010142/
https://www.ncbi.nlm.nih.gov/pubmed/575047
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author Welsch, C. W.
Dombroske, S. E.
McManus, M. J.
Calaf, G.
author_facet Welsch, C. W.
Dombroske, S. E.
McManus, M. J.
Calaf, G.
author_sort Welsch, C. W.
collection PubMed
description Ten benign breast tumours from 9 female patients (8 with fibrocystic disease and 1 with fibroadenoma) and 1 male patient (with gynaecomastia) were processed into slices and individually cultured for 2 days in serum-free Medium 199. [3H]-TdR was added to the culture medium to assess DNA synthesis. The addition of human prolactin to the culture medium (500 ng/ml) significantly (0.05 greater than P greater than 0.01) increased DNA synthesis; all 9 biopsy specimens from the 9 female patients responded positively to this hormone. Ovine prolactin (500 ng/ml) and bovine prolactin (500 ng/ml) increased the mean incorporation of [3H]-TdR into extracted DNA and increased the mean number of [3H]-TdR-labelled cells, but this increase did not reach the 5% level of probability. The sole case of male breast dysplasia analysed in this study did not respond to either human, ovine or bovine prolactin. These results provide evidence that human prolactin and, to a lesser degree, ovine and bovine prolactin are direct mitogenic stimulants to the epithelium in human (female) benign breast tumours.
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spelling pubmed-20101422009-09-10 Effect of human, bovine and ovine prolactin on DNA synthesis by organ cultures of benign human breast tumours. Welsch, C. W. Dombroske, S. E. McManus, M. J. Calaf, G. Br J Cancer Research Article Ten benign breast tumours from 9 female patients (8 with fibrocystic disease and 1 with fibroadenoma) and 1 male patient (with gynaecomastia) were processed into slices and individually cultured for 2 days in serum-free Medium 199. [3H]-TdR was added to the culture medium to assess DNA synthesis. The addition of human prolactin to the culture medium (500 ng/ml) significantly (0.05 greater than P greater than 0.01) increased DNA synthesis; all 9 biopsy specimens from the 9 female patients responded positively to this hormone. Ovine prolactin (500 ng/ml) and bovine prolactin (500 ng/ml) increased the mean incorporation of [3H]-TdR into extracted DNA and increased the mean number of [3H]-TdR-labelled cells, but this increase did not reach the 5% level of probability. The sole case of male breast dysplasia analysed in this study did not respond to either human, ovine or bovine prolactin. These results provide evidence that human prolactin and, to a lesser degree, ovine and bovine prolactin are direct mitogenic stimulants to the epithelium in human (female) benign breast tumours. Nature Publishing Group 1979-12 /pmc/articles/PMC2010142/ /pubmed/575047 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Welsch, C. W.
Dombroske, S. E.
McManus, M. J.
Calaf, G.
Effect of human, bovine and ovine prolactin on DNA synthesis by organ cultures of benign human breast tumours.
title Effect of human, bovine and ovine prolactin on DNA synthesis by organ cultures of benign human breast tumours.
title_full Effect of human, bovine and ovine prolactin on DNA synthesis by organ cultures of benign human breast tumours.
title_fullStr Effect of human, bovine and ovine prolactin on DNA synthesis by organ cultures of benign human breast tumours.
title_full_unstemmed Effect of human, bovine and ovine prolactin on DNA synthesis by organ cultures of benign human breast tumours.
title_short Effect of human, bovine and ovine prolactin on DNA synthesis by organ cultures of benign human breast tumours.
title_sort effect of human, bovine and ovine prolactin on dna synthesis by organ cultures of benign human breast tumours.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010142/
https://www.ncbi.nlm.nih.gov/pubmed/575047
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