Malignant myelomonocytic cells after in vitro infection of marrow cells with Friend leukaemia virus.

Infection of long-term BDF1 marrow cultures with Friend leukaemia virus complex (FLV) induced transformed cells with myelomonocytic characteristics, which were isolated only 14 days after the viral infection. Criteria for transformation were growth in suspension cultures and high plating efficiency...

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Autores principales: Testa, N. G., Dexter, T. M., Scott, D., Teich, N. M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1980
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010174/
https://www.ncbi.nlm.nih.gov/pubmed/6928787
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author Testa, N. G.
Dexter, T. M.
Scott, D.
Teich, N. M.
author_facet Testa, N. G.
Dexter, T. M.
Scott, D.
Teich, N. M.
author_sort Testa, N. G.
collection PubMed
description Infection of long-term BDF1 marrow cultures with Friend leukaemia virus complex (FLV) induced transformed cells with myelomonocytic characteristics, which were isolated only 14 days after the viral infection. Criteria for transformation were growth in suspension cultures and high plating efficiency in agar. The lymphatic leukaemia virus (LLV) replicates in these suspension cultures, but the spleen focus-forming virus (SFFV) component of the FLV complex has not been detected. Injection of the transformed cells into syngeneic neonatal or adult mice leads to the development of leukaemia which can be demonstrated to be of donor origin by the presence of two metacentric marker chromosomes which are also seen in the cultured cells. IMAGES:
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spelling pubmed-20101742009-09-10 Malignant myelomonocytic cells after in vitro infection of marrow cells with Friend leukaemia virus. Testa, N. G. Dexter, T. M. Scott, D. Teich, N. M. Br J Cancer Research Article Infection of long-term BDF1 marrow cultures with Friend leukaemia virus complex (FLV) induced transformed cells with myelomonocytic characteristics, which were isolated only 14 days after the viral infection. Criteria for transformation were growth in suspension cultures and high plating efficiency in agar. The lymphatic leukaemia virus (LLV) replicates in these suspension cultures, but the spleen focus-forming virus (SFFV) component of the FLV complex has not been detected. Injection of the transformed cells into syngeneic neonatal or adult mice leads to the development of leukaemia which can be demonstrated to be of donor origin by the presence of two metacentric marker chromosomes which are also seen in the cultured cells. IMAGES: Nature Publishing Group 1980-01 /pmc/articles/PMC2010174/ /pubmed/6928787 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Testa, N. G.
Dexter, T. M.
Scott, D.
Teich, N. M.
Malignant myelomonocytic cells after in vitro infection of marrow cells with Friend leukaemia virus.
title Malignant myelomonocytic cells after in vitro infection of marrow cells with Friend leukaemia virus.
title_full Malignant myelomonocytic cells after in vitro infection of marrow cells with Friend leukaemia virus.
title_fullStr Malignant myelomonocytic cells after in vitro infection of marrow cells with Friend leukaemia virus.
title_full_unstemmed Malignant myelomonocytic cells after in vitro infection of marrow cells with Friend leukaemia virus.
title_short Malignant myelomonocytic cells after in vitro infection of marrow cells with Friend leukaemia virus.
title_sort malignant myelomonocytic cells after in vitro infection of marrow cells with friend leukaemia virus.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010174/
https://www.ncbi.nlm.nih.gov/pubmed/6928787
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