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Evaluation of five DNA extraction methods for purification of DNA from atherosclerotic tissue and estimation of prevalence of Chlamydia pneumoniae in tissue from a Danish population undergoing vascular repair

BACKGROUND: To date PCR detection of Chlamydia pneumoniae DNA in atherosclerotic lesions from Danish patients has been unsuccessful. To establish whether non-detection was caused by a suboptimal DNA extraction method, we tested five different DNA extraction methods for purification of DNA from ather...

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Detalles Bibliográficos
Autores principales: Mygind, Tina, Østergaard, Lars, Birkelund, Svend, Lindholt, Jes S, Christiansen, Gunna
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC201026/
https://www.ncbi.nlm.nih.gov/pubmed/12952556
http://dx.doi.org/10.1186/1471-2180-3-19