Time dependence of the in vitro cytotoxicity of hexamethylmelamine and its metabolites.

The cytotoxicity of hexamethylmelamine (HMM) and its metabolites pentamethylmelamine (PMM), N,2,2,4,6-tetramethylmelamine (TMM) and hydroxymethylpentamethylmelamine (HMPMM) and of the alkylating agent triethylenemelamine (TEM) were studied on a cell line derived from a human ovarian cancer, by measu...

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Autores principales: D'Incalci, M., Erba, E., Balconi, G., Morasca, L., Garattini, S.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1980
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010283/
https://www.ncbi.nlm.nih.gov/pubmed/6770884
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author D'Incalci, M.
Erba, E.
Balconi, G.
Morasca, L.
Garattini, S.
author_facet D'Incalci, M.
Erba, E.
Balconi, G.
Morasca, L.
Garattini, S.
author_sort D'Incalci, M.
collection PubMed
description The cytotoxicity of hexamethylmelamine (HMM) and its metabolites pentamethylmelamine (PMM), N,2,2,4,6-tetramethylmelamine (TMM) and hydroxymethylpentamethylmelamine (HMPMM) and of the alkylating agent triethylenemelamine (TEM) were studied on a cell line derived from a human ovarian cancer, by measuring [3H]TdR uptake. After 24 h of incubation all the tested compounds inhibited [3H]TdR uptake, but only at a concentration of 100 micrograms/ml. However, after 120 h incubation, concentrations of 0.1--10 micrograms/ml resulted in highly significant cytotoxicity. HMPMM and TEM were the most active and their effect was not reversed 72 h after their removal. In our in vitro system no metabolism of HMM was observed.
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spelling pubmed-20102832009-09-10 Time dependence of the in vitro cytotoxicity of hexamethylmelamine and its metabolites. D'Incalci, M. Erba, E. Balconi, G. Morasca, L. Garattini, S. Br J Cancer Research Article The cytotoxicity of hexamethylmelamine (HMM) and its metabolites pentamethylmelamine (PMM), N,2,2,4,6-tetramethylmelamine (TMM) and hydroxymethylpentamethylmelamine (HMPMM) and of the alkylating agent triethylenemelamine (TEM) were studied on a cell line derived from a human ovarian cancer, by measuring [3H]TdR uptake. After 24 h of incubation all the tested compounds inhibited [3H]TdR uptake, but only at a concentration of 100 micrograms/ml. However, after 120 h incubation, concentrations of 0.1--10 micrograms/ml resulted in highly significant cytotoxicity. HMPMM and TEM were the most active and their effect was not reversed 72 h after their removal. In our in vitro system no metabolism of HMM was observed. Nature Publishing Group 1980-04 /pmc/articles/PMC2010283/ /pubmed/6770884 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
D'Incalci, M.
Erba, E.
Balconi, G.
Morasca, L.
Garattini, S.
Time dependence of the in vitro cytotoxicity of hexamethylmelamine and its metabolites.
title Time dependence of the in vitro cytotoxicity of hexamethylmelamine and its metabolites.
title_full Time dependence of the in vitro cytotoxicity of hexamethylmelamine and its metabolites.
title_fullStr Time dependence of the in vitro cytotoxicity of hexamethylmelamine and its metabolites.
title_full_unstemmed Time dependence of the in vitro cytotoxicity of hexamethylmelamine and its metabolites.
title_short Time dependence of the in vitro cytotoxicity of hexamethylmelamine and its metabolites.
title_sort time dependence of the in vitro cytotoxicity of hexamethylmelamine and its metabolites.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010283/
https://www.ncbi.nlm.nih.gov/pubmed/6770884
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AT morascal timedependenceoftheinvitrocytotoxicityofhexamethylmelamineanditsmetabolites
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