Further characterization of a melanoma-specific protein from human urine.

Isolation of a melanoma-specific protein (MSP) from human urine has been achieved using antibody affinity chromatography. MSP migrates as a single homogeneous protein on SDS PAGE and comparison of these data and ultracentrifuge analyses indicates that MSP contains a single polypeptide chain. MSP, ho...

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Detalles Bibliográficos
Autores principales: Bennett, C., Cooke, K. B.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1980
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010305/
https://www.ncbi.nlm.nih.gov/pubmed/6158965
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author Bennett, C.
Cooke, K. B.
author_facet Bennett, C.
Cooke, K. B.
author_sort Bennett, C.
collection PubMed
description Isolation of a melanoma-specific protein (MSP) from human urine has been achieved using antibody affinity chromatography. MSP migrates as a single homogeneous protein on SDS PAGE and comparison of these data and ultracentrifuge analyses indicates that MSP contains a single polypeptide chain. MSP, however, shows considerable charge heterogeneity on isoelectric focusing. The desialo form, alpha 2 MSP, is found predominantly in patients with advanced metastatic disease, whilst only the sialo form alpha 1 MSP, is obtained from the urine of patients with early-stage disease. MSP does not react with antisera raised to alpha 1 foetoprotein (AFP) or carcinoembryonic antigen (CEA) and hence is immunologically distinct from these other tumour-associated glycoproteins. Antisera raised to MSP do not react with normal skin melanocytes nor with any foetal tissue tested, and hence the origin of MSP remains unresolved. IMAGES:
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spelling pubmed-20103052009-09-10 Further characterization of a melanoma-specific protein from human urine. Bennett, C. Cooke, K. B. Br J Cancer Research Article Isolation of a melanoma-specific protein (MSP) from human urine has been achieved using antibody affinity chromatography. MSP migrates as a single homogeneous protein on SDS PAGE and comparison of these data and ultracentrifuge analyses indicates that MSP contains a single polypeptide chain. MSP, however, shows considerable charge heterogeneity on isoelectric focusing. The desialo form, alpha 2 MSP, is found predominantly in patients with advanced metastatic disease, whilst only the sialo form alpha 1 MSP, is obtained from the urine of patients with early-stage disease. MSP does not react with antisera raised to alpha 1 foetoprotein (AFP) or carcinoembryonic antigen (CEA) and hence is immunologically distinct from these other tumour-associated glycoproteins. Antisera raised to MSP do not react with normal skin melanocytes nor with any foetal tissue tested, and hence the origin of MSP remains unresolved. IMAGES: Nature Publishing Group 1980-05 /pmc/articles/PMC2010305/ /pubmed/6158965 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Bennett, C.
Cooke, K. B.
Further characterization of a melanoma-specific protein from human urine.
title Further characterization of a melanoma-specific protein from human urine.
title_full Further characterization of a melanoma-specific protein from human urine.
title_fullStr Further characterization of a melanoma-specific protein from human urine.
title_full_unstemmed Further characterization of a melanoma-specific protein from human urine.
title_short Further characterization of a melanoma-specific protein from human urine.
title_sort further characterization of a melanoma-specific protein from human urine.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010305/
https://www.ncbi.nlm.nih.gov/pubmed/6158965
work_keys_str_mv AT bennettc furthercharacterizationofamelanomaspecificproteinfromhumanurine
AT cookekb furthercharacterizationofamelanomaspecificproteinfromhumanurine

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