Human tumour karyology: marked analytic improvement by short-term agar culture.

A simple method has been developed which facilitates the detailed cytogenetic analysis of proliferating tumour cells within clusters and colonies arising from clonogenic tumour stem cells in biopsy samples of human cancers. The method uses a simple agar cloning technique for human tumours, which pro...

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Detalles Bibliográficos
Autores principales: Trent, J. M., Salmon, S. E.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1980
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010341/
https://www.ncbi.nlm.nih.gov/pubmed/7426311
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author Trent, J. M.
Salmon, S. E.
author_facet Trent, J. M.
Salmon, S. E.
author_sort Trent, J. M.
collection PubMed
description A simple method has been developed which facilitates the detailed cytogenetic analysis of proliferating tumour cells within clusters and colonies arising from clonogenic tumour stem cells in biopsy samples of human cancers. The method uses a simple agar cloning technique for human tumours, which provides marked enhancement in the number of cases with observable mitotic activity and the number of mitotic figures available for detailed karyotypic assessment. The frequency of mitotic figures in cluster and colony samples is much greater than is attainable with standard chromosomal techniques. This novel approach should prove to be a powerful tool for the study of human tumour karyology. IMAGES:
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spelling pubmed-20103412009-09-10 Human tumour karyology: marked analytic improvement by short-term agar culture. Trent, J. M. Salmon, S. E. Br J Cancer Research Article A simple method has been developed which facilitates the detailed cytogenetic analysis of proliferating tumour cells within clusters and colonies arising from clonogenic tumour stem cells in biopsy samples of human cancers. The method uses a simple agar cloning technique for human tumours, which provides marked enhancement in the number of cases with observable mitotic activity and the number of mitotic figures available for detailed karyotypic assessment. The frequency of mitotic figures in cluster and colony samples is much greater than is attainable with standard chromosomal techniques. This novel approach should prove to be a powerful tool for the study of human tumour karyology. IMAGES: Nature Publishing Group 1980-06 /pmc/articles/PMC2010341/ /pubmed/7426311 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Trent, J. M.
Salmon, S. E.
Human tumour karyology: marked analytic improvement by short-term agar culture.
title Human tumour karyology: marked analytic improvement by short-term agar culture.
title_full Human tumour karyology: marked analytic improvement by short-term agar culture.
title_fullStr Human tumour karyology: marked analytic improvement by short-term agar culture.
title_full_unstemmed Human tumour karyology: marked analytic improvement by short-term agar culture.
title_short Human tumour karyology: marked analytic improvement by short-term agar culture.
title_sort human tumour karyology: marked analytic improvement by short-term agar culture.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010341/
https://www.ncbi.nlm.nih.gov/pubmed/7426311
work_keys_str_mv AT trentjm humantumourkaryologymarkedanalyticimprovementbyshorttermagarculture
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