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Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival.
Multicellular tumour spheroids of the EMT6 mouse tumour line have been grown in a static (i.e. non-spinner) culture system to a mean spheroid diameter of 250 mum. Samples of spheroids were then exposed for 1 h to graded concentrations of various cytotoxic drugs, and the response assayed by both grow...
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1980
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010392/ https://www.ncbi.nlm.nih.gov/pubmed/7426337 |
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author | Twentyman, P. R. |
author_facet | Twentyman, P. R. |
author_sort | Twentyman, P. R. |
collection | PubMed |
description | Multicellular tumour spheroids of the EMT6 mouse tumour line have been grown in a static (i.e. non-spinner) culture system to a mean spheroid diameter of 250 mum. Samples of spheroids were then exposed for 1 h to graded concentrations of various cytotoxic drugs, and the response assayed by both growth delay and survival of clonogenic cells. For nitrogen mustard (HN2), melphalan, BCNU, CCNU and cis-platinum, a considerable recovery in measured cell survival was seen if correlation between growth delay and cell survival (measured at 24 h) was observed for these 5 agents. For adriamycin, actinomycin D and 5-fluorouracil, no increase in measured cell survival was seen for a 24h delay in assay, and these agents produced longer growth delays for a given level of cell kill. |
format | Text |
id | pubmed-2010392 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1980 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-20103922009-09-10 Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival. Twentyman, P. R. Br J Cancer Research Article Multicellular tumour spheroids of the EMT6 mouse tumour line have been grown in a static (i.e. non-spinner) culture system to a mean spheroid diameter of 250 mum. Samples of spheroids were then exposed for 1 h to graded concentrations of various cytotoxic drugs, and the response assayed by both growth delay and survival of clonogenic cells. For nitrogen mustard (HN2), melphalan, BCNU, CCNU and cis-platinum, a considerable recovery in measured cell survival was seen if correlation between growth delay and cell survival (measured at 24 h) was observed for these 5 agents. For adriamycin, actinomycin D and 5-fluorouracil, no increase in measured cell survival was seen for a 24h delay in assay, and these agents produced longer growth delays for a given level of cell kill. Nature Publishing Group 1980-08 /pmc/articles/PMC2010392/ /pubmed/7426337 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Twentyman, P. R. Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival. |
title | Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival. |
title_full | Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival. |
title_fullStr | Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival. |
title_full_unstemmed | Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival. |
title_short | Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival. |
title_sort | response to chemotherapy of emt6 spheroids as measured by growth delay and cell survival. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010392/ https://www.ncbi.nlm.nih.gov/pubmed/7426337 |
work_keys_str_mv | AT twentymanpr responsetochemotherapyofemt6spheroidsasmeasuredbygrowthdelayandcellsurvival |