Tumour-necrosis factor from the rabbit. IV. Purification and chemical characterization.

Serum from rabbits with BCG/endotoxin-induced shock is growth inhibitory or cytotoxic to a range ot tumour cell lines. The active component, tumour-necrosis factor (TNF), has been purified 1000-fold by sequential salt precipitation, ion-exchange chromatography and gel-filtration. TNF had a mol. wt o...

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Autores principales: Matthews, N., Ryley, H. C., Neale, M. L.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1980
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010414/
https://www.ncbi.nlm.nih.gov/pubmed/7426345
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author Matthews, N.
Ryley, H. C.
Neale, M. L.
author_facet Matthews, N.
Ryley, H. C.
Neale, M. L.
author_sort Matthews, N.
collection PubMed
description Serum from rabbits with BCG/endotoxin-induced shock is growth inhibitory or cytotoxic to a range ot tumour cell lines. The active component, tumour-necrosis factor (TNF), has been purified 1000-fold by sequential salt precipitation, ion-exchange chromatography and gel-filtration. TNF had a mol. wt of 67,000 on gradient PAGE and 39,000 on Ultrogel AcA44 gel-filtration. The isoelectric point was pH 5.1-5.2. TNF was susceptible to the proteolytic enzyme pronase, but resistant to trypsin or papain. On isopycnic ultracentrifugation it had a buoyant density of 1.27, confirming that it is protein in nature, with little or no carbohydrate. This is also suggested by its failure to bind to a range of lectins. IMAGES:
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spelling pubmed-20104142009-09-10 Tumour-necrosis factor from the rabbit. IV. Purification and chemical characterization. Matthews, N. Ryley, H. C. Neale, M. L. Br J Cancer Research Article Serum from rabbits with BCG/endotoxin-induced shock is growth inhibitory or cytotoxic to a range ot tumour cell lines. The active component, tumour-necrosis factor (TNF), has been purified 1000-fold by sequential salt precipitation, ion-exchange chromatography and gel-filtration. TNF had a mol. wt of 67,000 on gradient PAGE and 39,000 on Ultrogel AcA44 gel-filtration. The isoelectric point was pH 5.1-5.2. TNF was susceptible to the proteolytic enzyme pronase, but resistant to trypsin or papain. On isopycnic ultracentrifugation it had a buoyant density of 1.27, confirming that it is protein in nature, with little or no carbohydrate. This is also suggested by its failure to bind to a range of lectins. IMAGES: Nature Publishing Group 1980-09 /pmc/articles/PMC2010414/ /pubmed/7426345 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Matthews, N.
Ryley, H. C.
Neale, M. L.
Tumour-necrosis factor from the rabbit. IV. Purification and chemical characterization.
title Tumour-necrosis factor from the rabbit. IV. Purification and chemical characterization.
title_full Tumour-necrosis factor from the rabbit. IV. Purification and chemical characterization.
title_fullStr Tumour-necrosis factor from the rabbit. IV. Purification and chemical characterization.
title_full_unstemmed Tumour-necrosis factor from the rabbit. IV. Purification and chemical characterization.
title_short Tumour-necrosis factor from the rabbit. IV. Purification and chemical characterization.
title_sort tumour-necrosis factor from the rabbit. iv. purification and chemical characterization.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010414/
https://www.ncbi.nlm.nih.gov/pubmed/7426345
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