Colony formation by normal and malignant human B-lymphocytes.

A method is described that permits colony formation in culture by B lymphocytes from normal blood and from blood, marrow or lymph nodes of patients with myeloma or lymphoma. The method depends on: (1) exhaustively depleting cell suspensions of T lymphocytes, (2) a medium conditioned by T lymphocytes...

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Autores principales: Izaguirre, C. A., Minden, M. D., Howatson, A. F., McCulloch, E. A.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1980
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010422/
https://www.ncbi.nlm.nih.gov/pubmed/6968572
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author Izaguirre, C. A.
Minden, M. D.
Howatson, A. F.
McCulloch, E. A.
author_facet Izaguirre, C. A.
Minden, M. D.
Howatson, A. F.
McCulloch, E. A.
author_sort Izaguirre, C. A.
collection PubMed
description A method is described that permits colony formation in culture by B lymphocytes from normal blood and from blood, marrow or lymph nodes of patients with myeloma or lymphoma. The method depends on: (1) exhaustively depleting cell suspensions of T lymphocytes, (2) a medium conditioned by T lymphocytes in the presence of phytohaemagglutinin (PHA-TCM), and (3) irradiated autologous or homologous T lymphocytes. Under these conditions the assay is linear. Cellular development of B lymphocytes can be followed; differentiation to plasma cells is seen in cultures of cells from normal individuals and myeloma patients, but not lymphoma patients. Malignant B lymphocytes in culture produced immunoglobulin of the class identified in the patient's blood, or in freshly obtained cells. We conclude that the assay is suitable for studying the growth, differentiation and regulation of normal and malignant B lymphocytes in culture. IMAGES:
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spelling pubmed-20104222009-09-10 Colony formation by normal and malignant human B-lymphocytes. Izaguirre, C. A. Minden, M. D. Howatson, A. F. McCulloch, E. A. Br J Cancer Research Article A method is described that permits colony formation in culture by B lymphocytes from normal blood and from blood, marrow or lymph nodes of patients with myeloma or lymphoma. The method depends on: (1) exhaustively depleting cell suspensions of T lymphocytes, (2) a medium conditioned by T lymphocytes in the presence of phytohaemagglutinin (PHA-TCM), and (3) irradiated autologous or homologous T lymphocytes. Under these conditions the assay is linear. Cellular development of B lymphocytes can be followed; differentiation to plasma cells is seen in cultures of cells from normal individuals and myeloma patients, but not lymphoma patients. Malignant B lymphocytes in culture produced immunoglobulin of the class identified in the patient's blood, or in freshly obtained cells. We conclude that the assay is suitable for studying the growth, differentiation and regulation of normal and malignant B lymphocytes in culture. IMAGES: Nature Publishing Group 1980-09 /pmc/articles/PMC2010422/ /pubmed/6968572 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Izaguirre, C. A.
Minden, M. D.
Howatson, A. F.
McCulloch, E. A.
Colony formation by normal and malignant human B-lymphocytes.
title Colony formation by normal and malignant human B-lymphocytes.
title_full Colony formation by normal and malignant human B-lymphocytes.
title_fullStr Colony formation by normal and malignant human B-lymphocytes.
title_full_unstemmed Colony formation by normal and malignant human B-lymphocytes.
title_short Colony formation by normal and malignant human B-lymphocytes.
title_sort colony formation by normal and malignant human b-lymphocytes.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010422/
https://www.ncbi.nlm.nih.gov/pubmed/6968572
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