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Significant variation in mouse-skin aryl hydrocarbon hydroxylase inducibility as a function of the hair growth cycle.

An easy, rapid and improved technique for homogenizing whole skin is described. This technique consists of reducing skin to a powder in liquid N2 by using a metallic mortar, and homogenizing the powder in a Potter-Elvehjem tube. Using this homogenizing method, we have shown that skin AHH activity in...

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Autores principales: Manil, L., Van Cantfort, J., Lapière, C. M., Gielen, J. E.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1981
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010511/
https://www.ncbi.nlm.nih.gov/pubmed/7470383
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author Manil, L.
Van Cantfort, J.
Lapière, C. M.
Gielen, J. E.
author_facet Manil, L.
Van Cantfort, J.
Lapière, C. M.
Gielen, J. E.
author_sort Manil, L.
collection PubMed
description An easy, rapid and improved technique for homogenizing whole skin is described. This technique consists of reducing skin to a powder in liquid N2 by using a metallic mortar, and homogenizing the powder in a Potter-Elvehjem tube. Using this homogenizing method, we have shown that skin AHH activity in C57BL/6K and C3H/Ico mice can be induced by i.p. injected or topically applied methylcholanthrene during a defined period of the hair growth cycle, i.e. between the 8th and 14th days after depilation (Stage 6 of the anagen period). In each experimental model, there is an optimal methylcholanthrene concentration which yields a maximum induction. Topical methylcholanthrene is also responsible for a smaller aryl hydrocarbon hydroxylase (AHH) induction when the chemical is applied the same day that the club hairs are plucked. On the other hand, skin AHH activity is never induced by methylcholanthrene in DBA/2J mice, a genetically non-responsive strain. No clear-cut segregation of skin AHH inducibility levels is found among the offspring from the back-cross between (C57BL/6J X DBA/2J)F1 and non-inducible DBA/2J mice.
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spelling pubmed-20105112009-09-10 Significant variation in mouse-skin aryl hydrocarbon hydroxylase inducibility as a function of the hair growth cycle. Manil, L. Van Cantfort, J. Lapière, C. M. Gielen, J. E. Br J Cancer Research Article An easy, rapid and improved technique for homogenizing whole skin is described. This technique consists of reducing skin to a powder in liquid N2 by using a metallic mortar, and homogenizing the powder in a Potter-Elvehjem tube. Using this homogenizing method, we have shown that skin AHH activity in C57BL/6K and C3H/Ico mice can be induced by i.p. injected or topically applied methylcholanthrene during a defined period of the hair growth cycle, i.e. between the 8th and 14th days after depilation (Stage 6 of the anagen period). In each experimental model, there is an optimal methylcholanthrene concentration which yields a maximum induction. Topical methylcholanthrene is also responsible for a smaller aryl hydrocarbon hydroxylase (AHH) induction when the chemical is applied the same day that the club hairs are plucked. On the other hand, skin AHH activity is never induced by methylcholanthrene in DBA/2J mice, a genetically non-responsive strain. No clear-cut segregation of skin AHH inducibility levels is found among the offspring from the back-cross between (C57BL/6J X DBA/2J)F1 and non-inducible DBA/2J mice. Nature Publishing Group 1981-02 /pmc/articles/PMC2010511/ /pubmed/7470383 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Manil, L.
Van Cantfort, J.
Lapière, C. M.
Gielen, J. E.
Significant variation in mouse-skin aryl hydrocarbon hydroxylase inducibility as a function of the hair growth cycle.
title Significant variation in mouse-skin aryl hydrocarbon hydroxylase inducibility as a function of the hair growth cycle.
title_full Significant variation in mouse-skin aryl hydrocarbon hydroxylase inducibility as a function of the hair growth cycle.
title_fullStr Significant variation in mouse-skin aryl hydrocarbon hydroxylase inducibility as a function of the hair growth cycle.
title_full_unstemmed Significant variation in mouse-skin aryl hydrocarbon hydroxylase inducibility as a function of the hair growth cycle.
title_short Significant variation in mouse-skin aryl hydrocarbon hydroxylase inducibility as a function of the hair growth cycle.
title_sort significant variation in mouse-skin aryl hydrocarbon hydroxylase inducibility as a function of the hair growth cycle.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010511/
https://www.ncbi.nlm.nih.gov/pubmed/7470383
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