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Human myeloid leukaemic cell interactions in vitro with normal myeloid colonies.

To determine whether myeloid leukaemic cells could inhibit normal myeloid colony formation, leukaemic cells at concentrations ranging from 0.5 to 8 X 10(6)/ml were co-cultured in agar but separated by a 1 ml underlayer from 10(5) low-density (less than 1.077 g/ml) nonadherent normal marrow cells. In...

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Autores principales: Spitzer, G., Verma, D. S., Beran, M., Zander, A. R., Dicke, K. A., McCredie, K. B., Siegel, S., Tindle, S.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1981
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010528/
https://www.ncbi.nlm.nih.gov/pubmed/6970587
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author Spitzer, G.
Verma, D. S.
Beran, M.
Zander, A. R.
Dicke, K. A.
McCredie, K. B.
Siegel, S.
Tindle, S.
author_facet Spitzer, G.
Verma, D. S.
Beran, M.
Zander, A. R.
Dicke, K. A.
McCredie, K. B.
Siegel, S.
Tindle, S.
author_sort Spitzer, G.
collection PubMed
description To determine whether myeloid leukaemic cells could inhibit normal myeloid colony formation, leukaemic cells at concentrations ranging from 0.5 to 8 X 10(6)/ml were co-cultured in agar but separated by a 1 ml underlayer from 10(5) low-density (less than 1.077 g/ml) nonadherent normal marrow cells. Inhibition of normal-marrow myeloid colony formation occurred regularly at high cell concentrations (8 X 10(6)) at a leukaemic:normal cell ratio of 80:1. This suppression persisted with addition of indomethacin (10(-6)M). On the other hand, both low leukaemic cell numbers and irradiated leukaemic cells frequently stimulated normal colony growth. No inhibitor of colony growth could be detected in leukaemic-conditioned media, and absorption of colony-stimulating activity (CSA) with leukaemic cells improved CSA activity. These experiments point to the difficulty in unravelling the effect of leukaemic cells on normal haemopoiesis (both inhibitory and stimulatory) by in vitro agar culture.
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spelling pubmed-20105282009-09-10 Human myeloid leukaemic cell interactions in vitro with normal myeloid colonies. Spitzer, G. Verma, D. S. Beran, M. Zander, A. R. Dicke, K. A. McCredie, K. B. Siegel, S. Tindle, S. Br J Cancer Research Article To determine whether myeloid leukaemic cells could inhibit normal myeloid colony formation, leukaemic cells at concentrations ranging from 0.5 to 8 X 10(6)/ml were co-cultured in agar but separated by a 1 ml underlayer from 10(5) low-density (less than 1.077 g/ml) nonadherent normal marrow cells. Inhibition of normal-marrow myeloid colony formation occurred regularly at high cell concentrations (8 X 10(6)) at a leukaemic:normal cell ratio of 80:1. This suppression persisted with addition of indomethacin (10(-6)M). On the other hand, both low leukaemic cell numbers and irradiated leukaemic cells frequently stimulated normal colony growth. No inhibitor of colony growth could be detected in leukaemic-conditioned media, and absorption of colony-stimulating activity (CSA) with leukaemic cells improved CSA activity. These experiments point to the difficulty in unravelling the effect of leukaemic cells on normal haemopoiesis (both inhibitory and stimulatory) by in vitro agar culture. Nature Publishing Group 1981-02 /pmc/articles/PMC2010528/ /pubmed/6970587 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Spitzer, G.
Verma, D. S.
Beran, M.
Zander, A. R.
Dicke, K. A.
McCredie, K. B.
Siegel, S.
Tindle, S.
Human myeloid leukaemic cell interactions in vitro with normal myeloid colonies.
title Human myeloid leukaemic cell interactions in vitro with normal myeloid colonies.
title_full Human myeloid leukaemic cell interactions in vitro with normal myeloid colonies.
title_fullStr Human myeloid leukaemic cell interactions in vitro with normal myeloid colonies.
title_full_unstemmed Human myeloid leukaemic cell interactions in vitro with normal myeloid colonies.
title_short Human myeloid leukaemic cell interactions in vitro with normal myeloid colonies.
title_sort human myeloid leukaemic cell interactions in vitro with normal myeloid colonies.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010528/
https://www.ncbi.nlm.nih.gov/pubmed/6970587
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