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Cytotoxic effect in vivo of selected chemotherapeutic agents on synchronized murine fibrosarcoma cells.
The cytotoxic effects in vivo of single doses of either adriamycin (ADM), 1-beta-D-arabinofuranosylcytosine (Ara-C), bleomycin (BLM), cis-diamminedichloroplatinum (II) (cis-DDP), or cyclophosphamide (CY) on murine fibrosarcoma (FSa) cell populations were determined. Tumour cells were separated and s...
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1980
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010549/ https://www.ncbi.nlm.nih.gov/pubmed/6161629 |
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author | Grdina, D. J. Sigdestad, C. P. Peters, L. J. |
author_facet | Grdina, D. J. Sigdestad, C. P. Peters, L. J. |
author_sort | Grdina, D. J. |
collection | PubMed |
description | The cytotoxic effects in vivo of single doses of either adriamycin (ADM), 1-beta-D-arabinofuranosylcytosine (Ara-C), bleomycin (BLM), cis-diamminedichloroplatinum (II) (cis-DDP), or cyclophosphamide (CY) on murine fibrosarcoma (FSa) cell populations were determined. Tumour cells were separated and synchronized by centrifugal elutriation. Viable tumour cells from selected elutriator fractions were then injected i.v. into whole-body-irradiated mice. Twenty minutes later selected doses of ADM, Ara-C, BLM, cis-DDP or CY were administered to selected groups of these animals. Fourteen days later the mice were killed. Killing of injected tumour cells by each of the chemotherapeutic agents was evidenced by a reduction in the lung cells by each of the chemotherapeutic agents was evidenced by a reduction in the lung colonies per cell injected in treated animals. Under these conditions the response of FSa cells in vivo to the 5 drugs tested differed both qualitatively and quantitatively. Ara-C was S-phase-specific in toxicity. ADM, BLM, and cis-DDP were preferentially toxic to S, G2+M and G1 cells respectively. CY, a drug requiring bioactivation to form alkylating metabolites, was found to be equally toxic to G1 and G2+M enriched populations, but less effective in killing cell populations enriched with early-S cells. |
format | Text |
id | pubmed-2010549 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1980 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-20105492009-09-10 Cytotoxic effect in vivo of selected chemotherapeutic agents on synchronized murine fibrosarcoma cells. Grdina, D. J. Sigdestad, C. P. Peters, L. J. Br J Cancer Research Article The cytotoxic effects in vivo of single doses of either adriamycin (ADM), 1-beta-D-arabinofuranosylcytosine (Ara-C), bleomycin (BLM), cis-diamminedichloroplatinum (II) (cis-DDP), or cyclophosphamide (CY) on murine fibrosarcoma (FSa) cell populations were determined. Tumour cells were separated and synchronized by centrifugal elutriation. Viable tumour cells from selected elutriator fractions were then injected i.v. into whole-body-irradiated mice. Twenty minutes later selected doses of ADM, Ara-C, BLM, cis-DDP or CY were administered to selected groups of these animals. Fourteen days later the mice were killed. Killing of injected tumour cells by each of the chemotherapeutic agents was evidenced by a reduction in the lung cells by each of the chemotherapeutic agents was evidenced by a reduction in the lung colonies per cell injected in treated animals. Under these conditions the response of FSa cells in vivo to the 5 drugs tested differed both qualitatively and quantitatively. Ara-C was S-phase-specific in toxicity. ADM, BLM, and cis-DDP were preferentially toxic to S, G2+M and G1 cells respectively. CY, a drug requiring bioactivation to form alkylating metabolites, was found to be equally toxic to G1 and G2+M enriched populations, but less effective in killing cell populations enriched with early-S cells. Nature Publishing Group 1980-11 /pmc/articles/PMC2010549/ /pubmed/6161629 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Grdina, D. J. Sigdestad, C. P. Peters, L. J. Cytotoxic effect in vivo of selected chemotherapeutic agents on synchronized murine fibrosarcoma cells. |
title | Cytotoxic effect in vivo of selected chemotherapeutic agents on synchronized murine fibrosarcoma cells. |
title_full | Cytotoxic effect in vivo of selected chemotherapeutic agents on synchronized murine fibrosarcoma cells. |
title_fullStr | Cytotoxic effect in vivo of selected chemotherapeutic agents on synchronized murine fibrosarcoma cells. |
title_full_unstemmed | Cytotoxic effect in vivo of selected chemotherapeutic agents on synchronized murine fibrosarcoma cells. |
title_short | Cytotoxic effect in vivo of selected chemotherapeutic agents on synchronized murine fibrosarcoma cells. |
title_sort | cytotoxic effect in vivo of selected chemotherapeutic agents on synchronized murine fibrosarcoma cells. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010549/ https://www.ncbi.nlm.nih.gov/pubmed/6161629 |
work_keys_str_mv | AT grdinadj cytotoxiceffectinvivoofselectedchemotherapeuticagentsonsynchronizedmurinefibrosarcomacells AT sigdestadcp cytotoxiceffectinvivoofselectedchemotherapeuticagentsonsynchronizedmurinefibrosarcomacells AT peterslj cytotoxiceffectinvivoofselectedchemotherapeuticagentsonsynchronizedmurinefibrosarcomacells |