Characterization of gangliosides in human leucocytes.

Lipids extracted from leucocyte pellets with chloroform-methanol were applied to a DEAE-Sephadex column an the gangliosides were eluted with 0.2M sodium acetate in methanol. The eluate was desalted by Sephadex G-50 column chromatography. The purified ganglioside was spotted on the high-performance t...

Descripción completa

Detalles Bibliográficos
Autores principales: Tsuboyama, A., Takaku, F., Sakamoto, S., Kano, Y., Ariga, T., Miyatake, T.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1980
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010586/
https://www.ncbi.nlm.nih.gov/pubmed/6936052
_version_ 1782136353295695872
author Tsuboyama, A.
Takaku, F.
Sakamoto, S.
Kano, Y.
Ariga, T.
Miyatake, T.
author_facet Tsuboyama, A.
Takaku, F.
Sakamoto, S.
Kano, Y.
Ariga, T.
Miyatake, T.
author_sort Tsuboyama, A.
collection PubMed
description Lipids extracted from leucocyte pellets with chloroform-methanol were applied to a DEAE-Sephadex column an the gangliosides were eluted with 0.2M sodium acetate in methanol. The eluate was desalted by Sephadex G-50 column chromatography. The purified ganglioside was spotted on the high-performance than-layer plate. The plate was developed and sprayed with resorcinol reagents and heated. Seventeen bands of gangliosides were demonstrated in normal human leucocytes. The composition of these gangliosides was different in the various kinds of leucocytes. Amounts of GM3 ganglioside were apparently greater in normal lymphocytes and leukaemic cells than in granulocytes. Among the acute-leukaemic cells, some kinds of complex gangliosides were much more abundant in myelogenous cells than in lymphocytic cells. These changes in ganglioside composition are suggested as new biochemical markers for leukaemic cells. IMAGES:
format Text
id pubmed-2010586
institution National Center for Biotechnology Information
language English
publishDate 1980
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-20105862009-09-10 Characterization of gangliosides in human leucocytes. Tsuboyama, A. Takaku, F. Sakamoto, S. Kano, Y. Ariga, T. Miyatake, T. Br J Cancer Research Article Lipids extracted from leucocyte pellets with chloroform-methanol were applied to a DEAE-Sephadex column an the gangliosides were eluted with 0.2M sodium acetate in methanol. The eluate was desalted by Sephadex G-50 column chromatography. The purified ganglioside was spotted on the high-performance than-layer plate. The plate was developed and sprayed with resorcinol reagents and heated. Seventeen bands of gangliosides were demonstrated in normal human leucocytes. The composition of these gangliosides was different in the various kinds of leucocytes. Amounts of GM3 ganglioside were apparently greater in normal lymphocytes and leukaemic cells than in granulocytes. Among the acute-leukaemic cells, some kinds of complex gangliosides were much more abundant in myelogenous cells than in lymphocytic cells. These changes in ganglioside composition are suggested as new biochemical markers for leukaemic cells. IMAGES: Nature Publishing Group 1980-12 /pmc/articles/PMC2010586/ /pubmed/6936052 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Tsuboyama, A.
Takaku, F.
Sakamoto, S.
Kano, Y.
Ariga, T.
Miyatake, T.
Characterization of gangliosides in human leucocytes.
title Characterization of gangliosides in human leucocytes.
title_full Characterization of gangliosides in human leucocytes.
title_fullStr Characterization of gangliosides in human leucocytes.
title_full_unstemmed Characterization of gangliosides in human leucocytes.
title_short Characterization of gangliosides in human leucocytes.
title_sort characterization of gangliosides in human leucocytes.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010586/
https://www.ncbi.nlm.nih.gov/pubmed/6936052
work_keys_str_mv AT tsuboyamaa characterizationofgangliosidesinhumanleucocytes
AT takakuf characterizationofgangliosidesinhumanleucocytes
AT sakamotos characterizationofgangliosidesinhumanleucocytes
AT kanoy characterizationofgangliosidesinhumanleucocytes
AT arigat characterizationofgangliosidesinhumanleucocytes
AT miyataket characterizationofgangliosidesinhumanleucocytes

Ejemplares similares