Heterophile binding of human antibodies to glycoproteins of retroviruses.

The binding of human immunoglobulin to Type C viruses has been analysed by radioimmunoassay. The assay is a double-antibody, solid-phase RIA, which has been optimized and calibrated using rabbit and human anti-MuLV sera. It detects varying concentrations of IgG binding to HL-23-V-1, a human Type C v...

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Autor principal: Cardoso, E. A.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1981
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010640/
https://www.ncbi.nlm.nih.gov/pubmed/6263306
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author Cardoso, E. A.
author_facet Cardoso, E. A.
author_sort Cardoso, E. A.
collection PubMed
description The binding of human immunoglobulin to Type C viruses has been analysed by radioimmunoassay. The assay is a double-antibody, solid-phase RIA, which has been optimized and calibrated using rabbit and human anti-MuLV sera. It detects varying concentrations of IgG binding to HL-23-V-1, a human Type C virus isolate, in all of a large number of human sera tested. As judged by inhibition with nonspecific glycoproteins, heterophile antigens and pure saccharides, this binding is to the glycoside moiety of the virus-envelope glycoproteins, in agreement with other recent reports. Nonspecific binding of this type stringently restricts the interpretation which can be placed on these and earlier data in man concerning antibodies to Type C viruses. It does not however exclude the possibility that Type C viruses do occur in man and do elicit antibody therein. IMAGES:
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spelling pubmed-20106402009-09-10 Heterophile binding of human antibodies to glycoproteins of retroviruses. Cardoso, E. A. Br J Cancer Research Article The binding of human immunoglobulin to Type C viruses has been analysed by radioimmunoassay. The assay is a double-antibody, solid-phase RIA, which has been optimized and calibrated using rabbit and human anti-MuLV sera. It detects varying concentrations of IgG binding to HL-23-V-1, a human Type C virus isolate, in all of a large number of human sera tested. As judged by inhibition with nonspecific glycoproteins, heterophile antigens and pure saccharides, this binding is to the glycoside moiety of the virus-envelope glycoproteins, in agreement with other recent reports. Nonspecific binding of this type stringently restricts the interpretation which can be placed on these and earlier data in man concerning antibodies to Type C viruses. It does not however exclude the possibility that Type C viruses do occur in man and do elicit antibody therein. IMAGES: Nature Publishing Group 1981-04 /pmc/articles/PMC2010640/ /pubmed/6263306 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Cardoso, E. A.
Heterophile binding of human antibodies to glycoproteins of retroviruses.
title Heterophile binding of human antibodies to glycoproteins of retroviruses.
title_full Heterophile binding of human antibodies to glycoproteins of retroviruses.
title_fullStr Heterophile binding of human antibodies to glycoproteins of retroviruses.
title_full_unstemmed Heterophile binding of human antibodies to glycoproteins of retroviruses.
title_short Heterophile binding of human antibodies to glycoproteins of retroviruses.
title_sort heterophile binding of human antibodies to glycoproteins of retroviruses.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010640/
https://www.ncbi.nlm.nih.gov/pubmed/6263306
work_keys_str_mv AT cardosoea heterophilebindingofhumanantibodiestoglycoproteinsofretroviruses

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