Increased detection of mammary carcinoma cells in marrow smears using antisera to epithelial membrane antigen.

We have developed a technique for the immunocytochemical staining of marrow smears using antiserum to epithelial membrane antigen (EMA). This membrane component is confined to, but widely distributed in, epithelial tissues and tumours derived from them, and is strongly expressed by infiltrating brea...

Descripción completa

Detalles Bibliográficos
Autores principales: Dearnaley, D. P., Sloane, J. P., Ormerod, M. G., Steele, K., Coombes, R. C., Clink, H. M., Powles, T. J., Ford, H. T., Gazet, J. C., Neville, A. M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1981
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010665/
https://www.ncbi.nlm.nih.gov/pubmed/7020739
_version_ 1782136369598955520
author Dearnaley, D. P.
Sloane, J. P.
Ormerod, M. G.
Steele, K.
Coombes, R. C.
Clink, H. M.
Powles, T. J.
Ford, H. T.
Gazet, J. C.
Neville, A. M.
author_facet Dearnaley, D. P.
Sloane, J. P.
Ormerod, M. G.
Steele, K.
Coombes, R. C.
Clink, H. M.
Powles, T. J.
Ford, H. T.
Gazet, J. C.
Neville, A. M.
author_sort Dearnaley, D. P.
collection PubMed
description We have developed a technique for the immunocytochemical staining of marrow smears using antiserum to epithelial membrane antigen (EMA). This membrane component is confined to, but widely distributed in, epithelial tissues and tumours derived from them, and is strongly expressed by infiltrating breast carcinoma cells. Marrow aspirates from patients with both early and metastatic breast cancer have been examined, and the results of immunocytochemical staining compared with conventional cytology and histology. Staining with antiserum to EMA enabled us to detect small numbers of carcinoma cells, and increased the yield of positive samples. Furthermore, using this technique, we found malignant cells in the marrow of patients with primary breast cancer with no other evidence of metastatic disease. Thus immunocytochemical staining for EMA may be of value in the detection of micrometastases in patients with primary breast carcinoma. IMAGES:
format Text
id pubmed-2010665
institution National Center for Biotechnology Information
language English
publishDate 1981
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-20106652009-09-10 Increased detection of mammary carcinoma cells in marrow smears using antisera to epithelial membrane antigen. Dearnaley, D. P. Sloane, J. P. Ormerod, M. G. Steele, K. Coombes, R. C. Clink, H. M. Powles, T. J. Ford, H. T. Gazet, J. C. Neville, A. M. Br J Cancer Research Article We have developed a technique for the immunocytochemical staining of marrow smears using antiserum to epithelial membrane antigen (EMA). This membrane component is confined to, but widely distributed in, epithelial tissues and tumours derived from them, and is strongly expressed by infiltrating breast carcinoma cells. Marrow aspirates from patients with both early and metastatic breast cancer have been examined, and the results of immunocytochemical staining compared with conventional cytology and histology. Staining with antiserum to EMA enabled us to detect small numbers of carcinoma cells, and increased the yield of positive samples. Furthermore, using this technique, we found malignant cells in the marrow of patients with primary breast cancer with no other evidence of metastatic disease. Thus immunocytochemical staining for EMA may be of value in the detection of micrometastases in patients with primary breast carcinoma. IMAGES: Nature Publishing Group 1981-07 /pmc/articles/PMC2010665/ /pubmed/7020739 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Dearnaley, D. P.
Sloane, J. P.
Ormerod, M. G.
Steele, K.
Coombes, R. C.
Clink, H. M.
Powles, T. J.
Ford, H. T.
Gazet, J. C.
Neville, A. M.
Increased detection of mammary carcinoma cells in marrow smears using antisera to epithelial membrane antigen.
title Increased detection of mammary carcinoma cells in marrow smears using antisera to epithelial membrane antigen.
title_full Increased detection of mammary carcinoma cells in marrow smears using antisera to epithelial membrane antigen.
title_fullStr Increased detection of mammary carcinoma cells in marrow smears using antisera to epithelial membrane antigen.
title_full_unstemmed Increased detection of mammary carcinoma cells in marrow smears using antisera to epithelial membrane antigen.
title_short Increased detection of mammary carcinoma cells in marrow smears using antisera to epithelial membrane antigen.
title_sort increased detection of mammary carcinoma cells in marrow smears using antisera to epithelial membrane antigen.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010665/
https://www.ncbi.nlm.nih.gov/pubmed/7020739
work_keys_str_mv AT dearnaleydp increaseddetectionofmammarycarcinomacellsinmarrowsmearsusingantiseratoepithelialmembraneantigen
AT sloanejp increaseddetectionofmammarycarcinomacellsinmarrowsmearsusingantiseratoepithelialmembraneantigen
AT ormerodmg increaseddetectionofmammarycarcinomacellsinmarrowsmearsusingantiseratoepithelialmembraneantigen
AT steelek increaseddetectionofmammarycarcinomacellsinmarrowsmearsusingantiseratoepithelialmembraneantigen
AT coombesrc increaseddetectionofmammarycarcinomacellsinmarrowsmearsusingantiseratoepithelialmembraneantigen
AT clinkhm increaseddetectionofmammarycarcinomacellsinmarrowsmearsusingantiseratoepithelialmembraneantigen
AT powlestj increaseddetectionofmammarycarcinomacellsinmarrowsmearsusingantiseratoepithelialmembraneantigen
AT fordht increaseddetectionofmammarycarcinomacellsinmarrowsmearsusingantiseratoepithelialmembraneantigen
AT gazetjc increaseddetectionofmammarycarcinomacellsinmarrowsmearsusingantiseratoepithelialmembraneantigen
AT nevilleam increaseddetectionofmammarycarcinomacellsinmarrowsmearsusingantiseratoepithelialmembraneantigen

Ejemplares similares