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Antitumour reactions of monoclonal antibody against a human osteogenic-sarcoma cell line.

Monoclonal antibody against an osteogenic-sarcoma cell line (791T) was prepared by production and cloning of a somatic-cell hybrid between the mouse myeloma P3-NS1 and spleen cells from 791T-immunized mice. Three clones of hybridoma producing antibody against 791T, as detected by 125I-labelled Prote...

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Autores principales: Embleton, M. J., Gunn, B., Byers, V. S., Baldwin, R. W.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1981
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010673/
https://www.ncbi.nlm.nih.gov/pubmed/6941806
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author Embleton, M. J.
Gunn, B.
Byers, V. S.
Baldwin, R. W.
author_facet Embleton, M. J.
Gunn, B.
Byers, V. S.
Baldwin, R. W.
author_sort Embleton, M. J.
collection PubMed
description Monoclonal antibody against an osteogenic-sarcoma cell line (791T) was prepared by production and cloning of a somatic-cell hybrid between the mouse myeloma P3-NS1 and spleen cells from 791T-immunized mice. Three clones of hybridoma producing antibody against 791T, as detected by 125I-labelled Protein A binding, were tested against a range of normal and tumour cell targets to determine the pattern of expression of the antigen detected. The 3 clones had identical activity. They reacted strongly against 791T cells and another osteogenic sarcoma, 788T, and more weakly against a further 2 from a total panel of 10 osteogenic-sarcoma lines. The antibody was negative for fibroblasts from the donor of 791T, and for other fibroblasts, human red blood cells, human peripheral mononuclear cells and sheep red blood cells. When tested against a panel of unrelated tumours, they reacted against individual cell lines derived from carcinomas of colon, lung, bladder and cervix. These cross-reactions were not observed with other colon or lung carcinomas, and it is suggested that the antibody was reacting with a tumour-associated antigen expressed randomly on different tumour types, rather than specifically on osteogenic sarcomas.
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spelling pubmed-20106732009-09-10 Antitumour reactions of monoclonal antibody against a human osteogenic-sarcoma cell line. Embleton, M. J. Gunn, B. Byers, V. S. Baldwin, R. W. Br J Cancer Research Article Monoclonal antibody against an osteogenic-sarcoma cell line (791T) was prepared by production and cloning of a somatic-cell hybrid between the mouse myeloma P3-NS1 and spleen cells from 791T-immunized mice. Three clones of hybridoma producing antibody against 791T, as detected by 125I-labelled Protein A binding, were tested against a range of normal and tumour cell targets to determine the pattern of expression of the antigen detected. The 3 clones had identical activity. They reacted strongly against 791T cells and another osteogenic sarcoma, 788T, and more weakly against a further 2 from a total panel of 10 osteogenic-sarcoma lines. The antibody was negative for fibroblasts from the donor of 791T, and for other fibroblasts, human red blood cells, human peripheral mononuclear cells and sheep red blood cells. When tested against a panel of unrelated tumours, they reacted against individual cell lines derived from carcinomas of colon, lung, bladder and cervix. These cross-reactions were not observed with other colon or lung carcinomas, and it is suggested that the antibody was reacting with a tumour-associated antigen expressed randomly on different tumour types, rather than specifically on osteogenic sarcomas. Nature Publishing Group 1981-05 /pmc/articles/PMC2010673/ /pubmed/6941806 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Embleton, M. J.
Gunn, B.
Byers, V. S.
Baldwin, R. W.
Antitumour reactions of monoclonal antibody against a human osteogenic-sarcoma cell line.
title Antitumour reactions of monoclonal antibody against a human osteogenic-sarcoma cell line.
title_full Antitumour reactions of monoclonal antibody against a human osteogenic-sarcoma cell line.
title_fullStr Antitumour reactions of monoclonal antibody against a human osteogenic-sarcoma cell line.
title_full_unstemmed Antitumour reactions of monoclonal antibody against a human osteogenic-sarcoma cell line.
title_short Antitumour reactions of monoclonal antibody against a human osteogenic-sarcoma cell line.
title_sort antitumour reactions of monoclonal antibody against a human osteogenic-sarcoma cell line.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010673/
https://www.ncbi.nlm.nih.gov/pubmed/6941806
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