Human monoclonal antibodies to lung-cancer antigens.

Lymphocytes obtained from hilar and bronchial lymph nodes from 23 patients undergoing radical surgery for carcinoma of the bronchus were fused with established rat or mouse myeloma lines. 62% of the resultant hybrids were found to be secreting human Ig detected by a sensitive staphylococcal Protein...

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Detalles Bibliográficos
Autores principales: Sikora, K., Wright, R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1981
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010695/
https://www.ncbi.nlm.nih.gov/pubmed/7248154
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author Sikora, K.
Wright, R.
author_facet Sikora, K.
Wright, R.
author_sort Sikora, K.
collection PubMed
description Lymphocytes obtained from hilar and bronchial lymph nodes from 23 patients undergoing radical surgery for carcinoma of the bronchus were fused with established rat or mouse myeloma lines. 62% of the resultant hybrids were found to be secreting human Ig detected by a sensitive staphylococcal Protein A-coupled SRBC assay. Immunoglobulins synthesized by such hybrids were internally labelled with 3H-lysine and their antibody activity against a variety of membrane preparations determined. Nine monoclonal antibodies were found which bound to molecules on lung-cancer membranes and not on normal lung membranes from the same patient.
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spelling pubmed-20106952009-09-10 Human monoclonal antibodies to lung-cancer antigens. Sikora, K. Wright, R. Br J Cancer Research Article Lymphocytes obtained from hilar and bronchial lymph nodes from 23 patients undergoing radical surgery for carcinoma of the bronchus were fused with established rat or mouse myeloma lines. 62% of the resultant hybrids were found to be secreting human Ig detected by a sensitive staphylococcal Protein A-coupled SRBC assay. Immunoglobulins synthesized by such hybrids were internally labelled with 3H-lysine and their antibody activity against a variety of membrane preparations determined. Nine monoclonal antibodies were found which bound to molecules on lung-cancer membranes and not on normal lung membranes from the same patient. Nature Publishing Group 1981-05 /pmc/articles/PMC2010695/ /pubmed/7248154 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Sikora, K.
Wright, R.
Human monoclonal antibodies to lung-cancer antigens.
title Human monoclonal antibodies to lung-cancer antigens.
title_full Human monoclonal antibodies to lung-cancer antigens.
title_fullStr Human monoclonal antibodies to lung-cancer antigens.
title_full_unstemmed Human monoclonal antibodies to lung-cancer antigens.
title_short Human monoclonal antibodies to lung-cancer antigens.
title_sort human monoclonal antibodies to lung-cancer antigens.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010695/
https://www.ncbi.nlm.nih.gov/pubmed/7248154
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