Cell electrophoresis for diagnostic purposes. II. Critical evaluation of conventional cytopherometry.

Determination of the electrophoretic mobility of test cells has been widely used in an attempt to detect so-called lymphokines in a laboratory test for cancer, but operational difficulties are inherent in conventional cytopherometers. This study therefore investigates the technical and operational a...

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Detalles Bibliográficos
Autores principales: Hoffmann, W., Kaufmann, R., Steiner, R., Werner, W.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1981
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010696/
https://www.ncbi.nlm.nih.gov/pubmed/7248145
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author Hoffmann, W.
Kaufmann, R.
Steiner, R.
Werner, W.
author_facet Hoffmann, W.
Kaufmann, R.
Steiner, R.
Werner, W.
author_sort Hoffmann, W.
collection PubMed
description Determination of the electrophoretic mobility of test cells has been widely used in an attempt to detect so-called lymphokines in a laboratory test for cancer, but operational difficulties are inherent in conventional cytopherometers. This study therefore investigates the technical and operational aspects of cell electrophoresis, using the Zeiss cytopherometer; e.g. influence of electro-osmosis, focus uncertainty, movement due to convection and other sources of error. Implications and possible improvements in the test are discussed.
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spelling pubmed-20106962009-09-10 Cell electrophoresis for diagnostic purposes. II. Critical evaluation of conventional cytopherometry. Hoffmann, W. Kaufmann, R. Steiner, R. Werner, W. Br J Cancer Research Article Determination of the electrophoretic mobility of test cells has been widely used in an attempt to detect so-called lymphokines in a laboratory test for cancer, but operational difficulties are inherent in conventional cytopherometers. This study therefore investigates the technical and operational aspects of cell electrophoresis, using the Zeiss cytopherometer; e.g. influence of electro-osmosis, focus uncertainty, movement due to convection and other sources of error. Implications and possible improvements in the test are discussed. Nature Publishing Group 1981-05 /pmc/articles/PMC2010696/ /pubmed/7248145 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Hoffmann, W.
Kaufmann, R.
Steiner, R.
Werner, W.
Cell electrophoresis for diagnostic purposes. II. Critical evaluation of conventional cytopherometry.
title Cell electrophoresis for diagnostic purposes. II. Critical evaluation of conventional cytopherometry.
title_full Cell electrophoresis for diagnostic purposes. II. Critical evaluation of conventional cytopherometry.
title_fullStr Cell electrophoresis for diagnostic purposes. II. Critical evaluation of conventional cytopherometry.
title_full_unstemmed Cell electrophoresis for diagnostic purposes. II. Critical evaluation of conventional cytopherometry.
title_short Cell electrophoresis for diagnostic purposes. II. Critical evaluation of conventional cytopherometry.
title_sort cell electrophoresis for diagnostic purposes. ii. critical evaluation of conventional cytopherometry.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010696/
https://www.ncbi.nlm.nih.gov/pubmed/7248145
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