A comparison of adriamycin and mAMSA in vitro: cell lethality and SCE studies.

We have compared the actions of ADM and mAMSA in Chinese hamster V79 cells in vitro, using cell survival and sister-chromatid exchange as end-points. Equimolar concentrations of ADM and mAMSA show similar toxicities to exponentially growing cells, and both drugs are less effective in killing chronic...

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Detalles Bibliográficos
Autores principales: West, C., Stratford, I. J., Barrass, N., Smith, E.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1981
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010861/
https://www.ncbi.nlm.nih.gov/pubmed/6895696
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author West, C.
Stratford, I. J.
Barrass, N.
Smith, E.
author_facet West, C.
Stratford, I. J.
Barrass, N.
Smith, E.
author_sort West, C.
collection PubMed
description We have compared the actions of ADM and mAMSA in Chinese hamster V79 cells in vitro, using cell survival and sister-chromatid exchange as end-points. Equimolar concentrations of ADM and mAMSA show similar toxicities to exponentially growing cells, and both drugs are less effective in killing chronically hypoxic and plateau-phase cells. Cytotoxicity to thermotolerant cells (41 degrees C for 16 h previously) shows little difference from that for exponential cells. Pre-treating cells with misonidazole under hypoxic conditions reduces the toxicity of both ADM and mAMSA. In addition, an ADM-resistant Chinese hamster cell line, 77A-177, was cross-resistant to mAMSA. Finally, low equimolar sub-toxic doses of both drugs were found to cause similar increases in the levels of sister-chromatid exchanges in V79 cells. These results reveal no major difference in activity between ADM and mAMSA in vitro. IMAGES:
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spelling pubmed-20108612009-09-10 A comparison of adriamycin and mAMSA in vitro: cell lethality and SCE studies. West, C. Stratford, I. J. Barrass, N. Smith, E. Br J Cancer Research Article We have compared the actions of ADM and mAMSA in Chinese hamster V79 cells in vitro, using cell survival and sister-chromatid exchange as end-points. Equimolar concentrations of ADM and mAMSA show similar toxicities to exponentially growing cells, and both drugs are less effective in killing chronically hypoxic and plateau-phase cells. Cytotoxicity to thermotolerant cells (41 degrees C for 16 h previously) shows little difference from that for exponential cells. Pre-treating cells with misonidazole under hypoxic conditions reduces the toxicity of both ADM and mAMSA. In addition, an ADM-resistant Chinese hamster cell line, 77A-177, was cross-resistant to mAMSA. Finally, low equimolar sub-toxic doses of both drugs were found to cause similar increases in the levels of sister-chromatid exchanges in V79 cells. These results reveal no major difference in activity between ADM and mAMSA in vitro. IMAGES: Nature Publishing Group 1981-12 /pmc/articles/PMC2010861/ /pubmed/6895696 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
West, C.
Stratford, I. J.
Barrass, N.
Smith, E.
A comparison of adriamycin and mAMSA in vitro: cell lethality and SCE studies.
title A comparison of adriamycin and mAMSA in vitro: cell lethality and SCE studies.
title_full A comparison of adriamycin and mAMSA in vitro: cell lethality and SCE studies.
title_fullStr A comparison of adriamycin and mAMSA in vitro: cell lethality and SCE studies.
title_full_unstemmed A comparison of adriamycin and mAMSA in vitro: cell lethality and SCE studies.
title_short A comparison of adriamycin and mAMSA in vitro: cell lethality and SCE studies.
title_sort comparison of adriamycin and mamsa in vitro: cell lethality and sce studies.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010861/
https://www.ncbi.nlm.nih.gov/pubmed/6895696
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