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Do cell lines in vitro reflect the properties of the tumours of origin? A study of lines derived from human melanoma xenografts.

The characteristics of 7 human melanoma cell lines were compared with those of the xenografts from which they were established. The ultrastructure, melanin content, isozyme pattern and chromosome numbers of the cell lines were closely similar to those of the corresponding xenografts. The different c...

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Autores principales: Tveit, K. M., Pihl, A.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1981
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010864/
https://www.ncbi.nlm.nih.gov/pubmed/7326190
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author Tveit, K. M.
Pihl, A.
author_facet Tveit, K. M.
Pihl, A.
author_sort Tveit, K. M.
collection PubMed
description The characteristics of 7 human melanoma cell lines were compared with those of the xenografts from which they were established. The ultrastructure, melanin content, isozyme pattern and chromosome numbers of the cell lines were closely similar to those of the corresponding xenografts. The different cell lines gave rise to colonies in soft agar of size and morphology similar to the parent xenografts, and the plating efficiencies were clearly correlated. However, no correlation was found between the growth rates in vivo and either the doubling times and saturation densities in monolayer cultures or the plating efficiencies in soft agar. Moreover, one of the cell lines lost its tumorigenic ability upon establishment in culture. Thus, although the properties of the cell lines by and large reflected those of the parent xenografts, important inconsistencies were seen. The data emphasize that extrapolations from continuous cell lines in vitro to tumour cells in vivo are not necessarily valid. A high content of cellular fibronectin was correlated with a compact colony morphology in soft agar and rapid attachment and spreading on plastic. The growth rates and cellular morphology of the cell lines were strongly influenced by TPA, DMSO, retinoic acid and theophylline, but not by alpha-melanocyte-stimulating hormone. A murine cell line established from one of the xenografts grew in soft agar and produced sarcoma in mice. The malignant murine cells had arisen by transformation of murine stromal cells during the first subcultures in vitro, possibly caused by a factor produced by the human melanoma cells. IMAGES:
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spelling pubmed-20108642009-09-10 Do cell lines in vitro reflect the properties of the tumours of origin? A study of lines derived from human melanoma xenografts. Tveit, K. M. Pihl, A. Br J Cancer Research Article The characteristics of 7 human melanoma cell lines were compared with those of the xenografts from which they were established. The ultrastructure, melanin content, isozyme pattern and chromosome numbers of the cell lines were closely similar to those of the corresponding xenografts. The different cell lines gave rise to colonies in soft agar of size and morphology similar to the parent xenografts, and the plating efficiencies were clearly correlated. However, no correlation was found between the growth rates in vivo and either the doubling times and saturation densities in monolayer cultures or the plating efficiencies in soft agar. Moreover, one of the cell lines lost its tumorigenic ability upon establishment in culture. Thus, although the properties of the cell lines by and large reflected those of the parent xenografts, important inconsistencies were seen. The data emphasize that extrapolations from continuous cell lines in vitro to tumour cells in vivo are not necessarily valid. A high content of cellular fibronectin was correlated with a compact colony morphology in soft agar and rapid attachment and spreading on plastic. The growth rates and cellular morphology of the cell lines were strongly influenced by TPA, DMSO, retinoic acid and theophylline, but not by alpha-melanocyte-stimulating hormone. A murine cell line established from one of the xenografts grew in soft agar and produced sarcoma in mice. The malignant murine cells had arisen by transformation of murine stromal cells during the first subcultures in vitro, possibly caused by a factor produced by the human melanoma cells. IMAGES: Nature Publishing Group 1981-12 /pmc/articles/PMC2010864/ /pubmed/7326190 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Tveit, K. M.
Pihl, A.
Do cell lines in vitro reflect the properties of the tumours of origin? A study of lines derived from human melanoma xenografts.
title Do cell lines in vitro reflect the properties of the tumours of origin? A study of lines derived from human melanoma xenografts.
title_full Do cell lines in vitro reflect the properties of the tumours of origin? A study of lines derived from human melanoma xenografts.
title_fullStr Do cell lines in vitro reflect the properties of the tumours of origin? A study of lines derived from human melanoma xenografts.
title_full_unstemmed Do cell lines in vitro reflect the properties of the tumours of origin? A study of lines derived from human melanoma xenografts.
title_short Do cell lines in vitro reflect the properties of the tumours of origin? A study of lines derived from human melanoma xenografts.
title_sort do cell lines in vitro reflect the properties of the tumours of origin? a study of lines derived from human melanoma xenografts.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010864/
https://www.ncbi.nlm.nih.gov/pubmed/7326190
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