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Response of two mouse tumours to hyperthermia with CCNU or melphalan.

The in vivo response of B16 melanoma and Lewis lung carcinoma to combinations of hyperthermia and graded doses of CCNU or Melphalan was studied. To obtain dose-response curves and quantitative comparisons of different treatments, an agar-colony assay was used to measure survival of cells from excise...

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Autores principales: Joiner, M. C., Steel, G. G., Stephens, T. C.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1982
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010966/
https://www.ncbi.nlm.nih.gov/pubmed/7059461
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author Joiner, M. C.
Steel, G. G.
Stephens, T. C.
author_facet Joiner, M. C.
Steel, G. G.
Stephens, T. C.
author_sort Joiner, M. C.
collection PubMed
description The in vivo response of B16 melanoma and Lewis lung carcinoma to combinations of hyperthermia and graded doses of CCNU or Melphalan was studied. To obtain dose-response curves and quantitative comparisons of different treatments, an agar-colony assay was used to measure survival of cells from excised tumours. For heating experiments, the use of 2 tumours per animal, one heated and one not, allowed all other factors to be kept constant. When tumours were immersed in a water-bath at 43 degrees C for 1 h, Thermal Enhancement Ratios (TER) measured from the slopes of the dose-response curves were up to 1.6 for CCNU and 2.4 for Melphalan. Direct heat killing of about 1 decade was seen for 1 h at 43 degrees C. The anaesthetic Saffan also enhanced drug cell kill; the largest Dose Modifying Factor (2.7) was measured for Melphalan in the Lewis lung tumour. The duration of heating, and waterbath temperature, both influenced the enhancement of cell killing by CCNU, as did the time of excision of tumours between 0 and 3 1/2 h after treatment. There was no difference in effect between 3 1/2 and 24 h. The interaction between heat and CCNU varied if the interval between them was altered. The maximum effect was found if the heat and drug were given in close sequence.
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spelling pubmed-20109662009-09-10 Response of two mouse tumours to hyperthermia with CCNU or melphalan. Joiner, M. C. Steel, G. G. Stephens, T. C. Br J Cancer Research Article The in vivo response of B16 melanoma and Lewis lung carcinoma to combinations of hyperthermia and graded doses of CCNU or Melphalan was studied. To obtain dose-response curves and quantitative comparisons of different treatments, an agar-colony assay was used to measure survival of cells from excised tumours. For heating experiments, the use of 2 tumours per animal, one heated and one not, allowed all other factors to be kept constant. When tumours were immersed in a water-bath at 43 degrees C for 1 h, Thermal Enhancement Ratios (TER) measured from the slopes of the dose-response curves were up to 1.6 for CCNU and 2.4 for Melphalan. Direct heat killing of about 1 decade was seen for 1 h at 43 degrees C. The anaesthetic Saffan also enhanced drug cell kill; the largest Dose Modifying Factor (2.7) was measured for Melphalan in the Lewis lung tumour. The duration of heating, and waterbath temperature, both influenced the enhancement of cell killing by CCNU, as did the time of excision of tumours between 0 and 3 1/2 h after treatment. There was no difference in effect between 3 1/2 and 24 h. The interaction between heat and CCNU varied if the interval between them was altered. The maximum effect was found if the heat and drug were given in close sequence. Nature Publishing Group 1982-01 /pmc/articles/PMC2010966/ /pubmed/7059461 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Joiner, M. C.
Steel, G. G.
Stephens, T. C.
Response of two mouse tumours to hyperthermia with CCNU or melphalan.
title Response of two mouse tumours to hyperthermia with CCNU or melphalan.
title_full Response of two mouse tumours to hyperthermia with CCNU or melphalan.
title_fullStr Response of two mouse tumours to hyperthermia with CCNU or melphalan.
title_full_unstemmed Response of two mouse tumours to hyperthermia with CCNU or melphalan.
title_short Response of two mouse tumours to hyperthermia with CCNU or melphalan.
title_sort response of two mouse tumours to hyperthermia with ccnu or melphalan.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010966/
https://www.ncbi.nlm.nih.gov/pubmed/7059461
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