Syngeneic antitumour antibodies in rats: clearance of cell-bound antibody in vivo and in vitro.

Hooded Lister/Cbi rats bearing the HSN.TC fibrosarcoma produced a high-titre non-complement-binding IgG antibody, and tests in vitro indicated that the syngeneic antibody was specific for this tumour. About 1.4 X 10(5) antibody molecules were bound per cell, a figure one eighth that for cells treate...

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Autores principales: Dean, C. J., Hobbs, S. M., Hopkins, J. U., North, S. M., Styles, J. M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1982
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011097/
https://www.ncbi.nlm.nih.gov/pubmed/7150472
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author Dean, C. J.
Hobbs, S. M.
Hopkins, J. U.
North, S. M.
Styles, J. M.
author_facet Dean, C. J.
Hobbs, S. M.
Hopkins, J. U.
North, S. M.
Styles, J. M.
author_sort Dean, C. J.
collection PubMed
description Hooded Lister/Cbi rats bearing the HSN.TC fibrosarcoma produced a high-titre non-complement-binding IgG antibody, and tests in vitro indicated that the syngeneic antibody was specific for this tumour. About 1.4 X 10(5) antibody molecules were bound per cell, a figure one eighth that for cells treated with a high-titre allo-antiserum. When tumour-bearer serum was passively transferred into congenitally athymic rats bearing the HSN.TC tumour the antibody was absorbed out specifically, by comparison with control animals or athymic rats bearing an unrelated tumour that was also syngeneic in Hooded rats. The kinetics of loss of antibody from the surface of HSN.TC cells has been monitored in vitro and the antibody has been found to have an extended half-life at the cell surface (greater than 40 h).
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spelling pubmed-20110972009-09-10 Syngeneic antitumour antibodies in rats: clearance of cell-bound antibody in vivo and in vitro. Dean, C. J. Hobbs, S. M. Hopkins, J. U. North, S. M. Styles, J. M. Br J Cancer Research Article Hooded Lister/Cbi rats bearing the HSN.TC fibrosarcoma produced a high-titre non-complement-binding IgG antibody, and tests in vitro indicated that the syngeneic antibody was specific for this tumour. About 1.4 X 10(5) antibody molecules were bound per cell, a figure one eighth that for cells treated with a high-titre allo-antiserum. When tumour-bearer serum was passively transferred into congenitally athymic rats bearing the HSN.TC tumour the antibody was absorbed out specifically, by comparison with control animals or athymic rats bearing an unrelated tumour that was also syngeneic in Hooded rats. The kinetics of loss of antibody from the surface of HSN.TC cells has been monitored in vitro and the antibody has been found to have an extended half-life at the cell surface (greater than 40 h). Nature Publishing Group 1982-08 /pmc/articles/PMC2011097/ /pubmed/7150472 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Dean, C. J.
Hobbs, S. M.
Hopkins, J. U.
North, S. M.
Styles, J. M.
Syngeneic antitumour antibodies in rats: clearance of cell-bound antibody in vivo and in vitro.
title Syngeneic antitumour antibodies in rats: clearance of cell-bound antibody in vivo and in vitro.
title_full Syngeneic antitumour antibodies in rats: clearance of cell-bound antibody in vivo and in vitro.
title_fullStr Syngeneic antitumour antibodies in rats: clearance of cell-bound antibody in vivo and in vitro.
title_full_unstemmed Syngeneic antitumour antibodies in rats: clearance of cell-bound antibody in vivo and in vitro.
title_short Syngeneic antitumour antibodies in rats: clearance of cell-bound antibody in vivo and in vitro.
title_sort syngeneic antitumour antibodies in rats: clearance of cell-bound antibody in vivo and in vitro.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011097/
https://www.ncbi.nlm.nih.gov/pubmed/7150472
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