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In vitro cytotoxic drug sensitivity testing of human tumour xenografts grown as multicellular tumour spheroids.
Tumour cells from 7 patients with ovarian carcinoma and from 22 different human tumour xenografts representing a wide range of histological sub-types have been examined for multicellular spheroid forming ability. Spheroid formation was limited to cells derived from xenografts. Of the 22 lines tested...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1982
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011214/ https://www.ncbi.nlm.nih.gov/pubmed/6891260 |
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author | Jones, A. C. Stratford, I. J. Wilson, P. A. Peckham, M. J. |
author_facet | Jones, A. C. Stratford, I. J. Wilson, P. A. Peckham, M. J. |
author_sort | Jones, A. C. |
collection | PubMed |
description | Tumour cells from 7 patients with ovarian carcinoma and from 22 different human tumour xenografts representing a wide range of histological sub-types have been examined for multicellular spheroid forming ability. Spheroid formation was limited to cells derived from xenografts. Of the 22 lines tested, 5 formed spheroids capable of growth in isolation. There was no clear relationship between histological type and spheroid-forming ability. The plating efficiency of tumour cells obtained from spheroids was always greater than for the cells obtained from the dissociated tumour of origin and was in some cases as much as 6-fold greater. Spheroid growth was nearly exponential for 4 cell lines. Volume growth delay was used to investigate the activity of melphalan, adriamycin, the Vinca alkaloids, CCNU and cisplatin. Differences between lines in drug response broadly reflected patient and in vivo xenograft response. |
format | Text |
id | pubmed-2011214 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1982 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-20112142009-09-10 In vitro cytotoxic drug sensitivity testing of human tumour xenografts grown as multicellular tumour spheroids. Jones, A. C. Stratford, I. J. Wilson, P. A. Peckham, M. J. Br J Cancer Research Article Tumour cells from 7 patients with ovarian carcinoma and from 22 different human tumour xenografts representing a wide range of histological sub-types have been examined for multicellular spheroid forming ability. Spheroid formation was limited to cells derived from xenografts. Of the 22 lines tested, 5 formed spheroids capable of growth in isolation. There was no clear relationship between histological type and spheroid-forming ability. The plating efficiency of tumour cells obtained from spheroids was always greater than for the cells obtained from the dissociated tumour of origin and was in some cases as much as 6-fold greater. Spheroid growth was nearly exponential for 4 cell lines. Volume growth delay was used to investigate the activity of melphalan, adriamycin, the Vinca alkaloids, CCNU and cisplatin. Differences between lines in drug response broadly reflected patient and in vivo xenograft response. Nature Publishing Group 1982-12 /pmc/articles/PMC2011214/ /pubmed/6891260 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Jones, A. C. Stratford, I. J. Wilson, P. A. Peckham, M. J. In vitro cytotoxic drug sensitivity testing of human tumour xenografts grown as multicellular tumour spheroids. |
title | In vitro cytotoxic drug sensitivity testing of human tumour xenografts grown as multicellular tumour spheroids. |
title_full | In vitro cytotoxic drug sensitivity testing of human tumour xenografts grown as multicellular tumour spheroids. |
title_fullStr | In vitro cytotoxic drug sensitivity testing of human tumour xenografts grown as multicellular tumour spheroids. |
title_full_unstemmed | In vitro cytotoxic drug sensitivity testing of human tumour xenografts grown as multicellular tumour spheroids. |
title_short | In vitro cytotoxic drug sensitivity testing of human tumour xenografts grown as multicellular tumour spheroids. |
title_sort | in vitro cytotoxic drug sensitivity testing of human tumour xenografts grown as multicellular tumour spheroids. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011214/ https://www.ncbi.nlm.nih.gov/pubmed/6891260 |
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