Cell aggregates in the soft agar "human tumour stem-cell assay".

We evaluated colony formation in soft agar by cells obtained after mechanical and/or enzymatic disaggregation of 455 malignant human tumours. Counting and assessment of cell colonies in the agar plates were done by inverted microscopy, computerized image analysis, and inspection of serial photomicro...

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Detalles Bibliográficos
Autores principales: Agrez, M. V., Kovach, J. S., Lieber, M. M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1982
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011235/
https://www.ncbi.nlm.nih.gov/pubmed/6295424
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author Agrez, M. V.
Kovach, J. S.
Lieber, M. M.
author_facet Agrez, M. V.
Kovach, J. S.
Lieber, M. M.
author_sort Agrez, M. V.
collection PubMed
description We evaluated colony formation in soft agar by cells obtained after mechanical and/or enzymatic disaggregation of 455 malignant human tumours. Counting and assessment of cell colonies in the agar plates were done by inverted microscopy, computerized image analysis, and inspection of serial photomicrographs of the agar plates. Our results indicate that standard methods of tumour disaggregation did not usually produce single-cell suspensions and that aggregates of tumour cells varying greatly in size were placed in the agar. Most groupings of cells identified as colonies 1-3 weeks after plating arose from enlargement of preexisting aggregates of cells. IMAGES:
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spelling pubmed-20112352009-09-10 Cell aggregates in the soft agar "human tumour stem-cell assay". Agrez, M. V. Kovach, J. S. Lieber, M. M. Br J Cancer Research Article We evaluated colony formation in soft agar by cells obtained after mechanical and/or enzymatic disaggregation of 455 malignant human tumours. Counting and assessment of cell colonies in the agar plates were done by inverted microscopy, computerized image analysis, and inspection of serial photomicrographs of the agar plates. Our results indicate that standard methods of tumour disaggregation did not usually produce single-cell suspensions and that aggregates of tumour cells varying greatly in size were placed in the agar. Most groupings of cells identified as colonies 1-3 weeks after plating arose from enlargement of preexisting aggregates of cells. IMAGES: Nature Publishing Group 1982-12 /pmc/articles/PMC2011235/ /pubmed/6295424 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Agrez, M. V.
Kovach, J. S.
Lieber, M. M.
Cell aggregates in the soft agar "human tumour stem-cell assay".
title Cell aggregates in the soft agar "human tumour stem-cell assay".
title_full Cell aggregates in the soft agar "human tumour stem-cell assay".
title_fullStr Cell aggregates in the soft agar "human tumour stem-cell assay".
title_full_unstemmed Cell aggregates in the soft agar "human tumour stem-cell assay".
title_short Cell aggregates in the soft agar "human tumour stem-cell assay".
title_sort cell aggregates in the soft agar "human tumour stem-cell assay".
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011235/
https://www.ncbi.nlm.nih.gov/pubmed/6295424
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