Human hybridomas from patients with malignant disease.

Lymphocytes from 180 patients with a variety of malignant diseases were collected and fused with a human myeloma-derived line, LON-LICR-HMy2/CAM1. A total of 162 hybridomas was obtained. Only B lymphocyte markers were found on the surface of the fusion products. Flow cytometric analysis revealed a s...

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Detalles Bibliográficos
Autores principales: Sikora, K., Alderson, T., Ellis, J., Phillips, J., Watson, J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1983
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011244/
https://www.ncbi.nlm.nih.gov/pubmed/6336943
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author Sikora, K.
Alderson, T.
Ellis, J.
Phillips, J.
Watson, J.
author_facet Sikora, K.
Alderson, T.
Ellis, J.
Phillips, J.
Watson, J.
author_sort Sikora, K.
collection PubMed
description Lymphocytes from 180 patients with a variety of malignant diseases were collected and fused with a human myeloma-derived line, LON-LICR-HMy2/CAM1. A total of 162 hybridomas was obtained. Only B lymphocyte markers were found on the surface of the fusion products. Flow cytometric analysis revealed a stably increased DNA content in the hybridoma cells. Some hybridoma supernatants were found to contain new Ig chains. Anti-tumour binding activity was found in 12 supernatants. IMAGES:
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spelling pubmed-20112442009-09-10 Human hybridomas from patients with malignant disease. Sikora, K. Alderson, T. Ellis, J. Phillips, J. Watson, J. Br J Cancer Research Article Lymphocytes from 180 patients with a variety of malignant diseases were collected and fused with a human myeloma-derived line, LON-LICR-HMy2/CAM1. A total of 162 hybridomas was obtained. Only B lymphocyte markers were found on the surface of the fusion products. Flow cytometric analysis revealed a stably increased DNA content in the hybridoma cells. Some hybridoma supernatants were found to contain new Ig chains. Anti-tumour binding activity was found in 12 supernatants. IMAGES: Nature Publishing Group 1983-01 /pmc/articles/PMC2011244/ /pubmed/6336943 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Sikora, K.
Alderson, T.
Ellis, J.
Phillips, J.
Watson, J.
Human hybridomas from patients with malignant disease.
title Human hybridomas from patients with malignant disease.
title_full Human hybridomas from patients with malignant disease.
title_fullStr Human hybridomas from patients with malignant disease.
title_full_unstemmed Human hybridomas from patients with malignant disease.
title_short Human hybridomas from patients with malignant disease.
title_sort human hybridomas from patients with malignant disease.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011244/
https://www.ncbi.nlm.nih.gov/pubmed/6336943
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