Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody.

Rat monoclonal antibodies were prepared by immunising rats with human colorectal carcinoma cell membranes and fusing splenic lymphocytes with a rat myeloma. Hybridoma supernatants were screened by binding assays on membranes prepared from colorectal carcinoma tissue. One hybridoma supernatant, conta...

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Detalles Bibliográficos
Autores principales: Smedley, H. M., Finan, P., Lennox, E. S., Ritson, A., Takei, F., Wraight, P., Sikora, K.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1983
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011266/
https://www.ncbi.nlm.nih.gov/pubmed/6337613
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author Smedley, H. M.
Finan, P.
Lennox, E. S.
Ritson, A.
Takei, F.
Wraight, P.
Sikora, K.
author_facet Smedley, H. M.
Finan, P.
Lennox, E. S.
Ritson, A.
Takei, F.
Wraight, P.
Sikora, K.
author_sort Smedley, H. M.
collection PubMed
description Rat monoclonal antibodies were prepared by immunising rats with human colorectal carcinoma cell membranes and fusing splenic lymphocytes with a rat myeloma. Hybridoma supernatants were screened by binding assays on membranes prepared from colorectal carcinoma tissue. One hybridoma supernatant, containing a monoclonal antibody with high binding activity on malignant compared to normal colon sections, was grown in large quantities in serum-free medium. After ammonium sulphate precipitation the antibody was purified by ion-exchange chromatography and labelled with 131I. Radiolabelled antibody was administered i.v. to 27 patients with colonic and other tumours. Scintigrams were obtained at 48 h. Computerised subtraction of the blood pool image revealed localised areas of uptake corresponding with areas of known disease in 13/16 patients with colorectal carcinoma and 3/4 patients with breast cancer. IMAGES:
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spelling pubmed-20112662009-09-10 Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody. Smedley, H. M. Finan, P. Lennox, E. S. Ritson, A. Takei, F. Wraight, P. Sikora, K. Br J Cancer Research Article Rat monoclonal antibodies were prepared by immunising rats with human colorectal carcinoma cell membranes and fusing splenic lymphocytes with a rat myeloma. Hybridoma supernatants were screened by binding assays on membranes prepared from colorectal carcinoma tissue. One hybridoma supernatant, containing a monoclonal antibody with high binding activity on malignant compared to normal colon sections, was grown in large quantities in serum-free medium. After ammonium sulphate precipitation the antibody was purified by ion-exchange chromatography and labelled with 131I. Radiolabelled antibody was administered i.v. to 27 patients with colonic and other tumours. Scintigrams were obtained at 48 h. Computerised subtraction of the blood pool image revealed localised areas of uptake corresponding with areas of known disease in 13/16 patients with colorectal carcinoma and 3/4 patients with breast cancer. IMAGES: Nature Publishing Group 1983-02 /pmc/articles/PMC2011266/ /pubmed/6337613 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Smedley, H. M.
Finan, P.
Lennox, E. S.
Ritson, A.
Takei, F.
Wraight, P.
Sikora, K.
Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody.
title Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody.
title_full Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody.
title_fullStr Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody.
title_full_unstemmed Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody.
title_short Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody.
title_sort localisation of metastatic carcinoma by a radiolabelled monoclonal antibody.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011266/
https://www.ncbi.nlm.nih.gov/pubmed/6337613
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