Characterization of human colon carcinoma cell lines isolated from a single primary tumour.

The initiation of a cultured human colon carcinoma line on a feeder layer of confluent fibroblasts is described. Attempts to initiate cultures without fibroblast feeder layers were not successful. Two sub-lines (designated HCT C and HCT C Col) were isolated and weaned from cells growing on the surfa...

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Autores principales: Brattain, M. G., Marks, M. E., McCombs, J., Finely, W., Brattain, D. E.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1983
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011309/
https://www.ncbi.nlm.nih.gov/pubmed/6830688
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author Brattain, M. G.
Marks, M. E.
McCombs, J.
Finely, W.
Brattain, D. E.
author_facet Brattain, M. G.
Marks, M. E.
McCombs, J.
Finely, W.
Brattain, D. E.
author_sort Brattain, M. G.
collection PubMed
description The initiation of a cultured human colon carcinoma line on a feeder layer of confluent fibroblasts is described. Attempts to initiate cultures without fibroblast feeder layers were not successful. Two sub-lines (designated HCT C and HCT C Col) were isolated and weaned from cells growing on the surface of the feeder layer. The sub-lines had different morphologies, secreted different levels of carcinoembryonic antigen (CEA) into the medium of confluent cultures and had slightly different karyotypes. Both sub-lines grew in semi-solid medium and formed xenografts when injected s.c. into athymic nude mice. Analysis of radioiodinated cell membrane components indicated small, but significant differences between the sub-lines. IMAGES:
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spelling pubmed-20113092009-09-10 Characterization of human colon carcinoma cell lines isolated from a single primary tumour. Brattain, M. G. Marks, M. E. McCombs, J. Finely, W. Brattain, D. E. Br J Cancer Research Article The initiation of a cultured human colon carcinoma line on a feeder layer of confluent fibroblasts is described. Attempts to initiate cultures without fibroblast feeder layers were not successful. Two sub-lines (designated HCT C and HCT C Col) were isolated and weaned from cells growing on the surface of the feeder layer. The sub-lines had different morphologies, secreted different levels of carcinoembryonic antigen (CEA) into the medium of confluent cultures and had slightly different karyotypes. Both sub-lines grew in semi-solid medium and formed xenografts when injected s.c. into athymic nude mice. Analysis of radioiodinated cell membrane components indicated small, but significant differences between the sub-lines. IMAGES: Nature Publishing Group 1983-03 /pmc/articles/PMC2011309/ /pubmed/6830688 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Brattain, M. G.
Marks, M. E.
McCombs, J.
Finely, W.
Brattain, D. E.
Characterization of human colon carcinoma cell lines isolated from a single primary tumour.
title Characterization of human colon carcinoma cell lines isolated from a single primary tumour.
title_full Characterization of human colon carcinoma cell lines isolated from a single primary tumour.
title_fullStr Characterization of human colon carcinoma cell lines isolated from a single primary tumour.
title_full_unstemmed Characterization of human colon carcinoma cell lines isolated from a single primary tumour.
title_short Characterization of human colon carcinoma cell lines isolated from a single primary tumour.
title_sort characterization of human colon carcinoma cell lines isolated from a single primary tumour.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011309/
https://www.ncbi.nlm.nih.gov/pubmed/6830688
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