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Effect of enzymic removal of cell surface constituents on metastatic colonisation potential of mouse mammary tumour cells.
Trypsin treatment of viable cells from 24 spontaneous murine mammary carcinomas resulted in a mild but reproducible diminution in their capability to colonise the lung after i.v. reinoculation but did not alter the distribution of deposits formed. The effects were similar on tumours of high and of l...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1983
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011500/ https://www.ncbi.nlm.nih.gov/pubmed/6626455 |
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author | Sargent, N. S. Price, J. E. Tarin, D. |
author_facet | Sargent, N. S. Price, J. E. Tarin, D. |
author_sort | Sargent, N. S. |
collection | PubMed |
description | Trypsin treatment of viable cells from 24 spontaneous murine mammary carcinomas resulted in a mild but reproducible diminution in their capability to colonise the lung after i.v. reinoculation but did not alter the distribution of deposits formed. The effects were similar on tumours of high and of low colonisation potentials. Neuraminidase and hyaluronidase did not exert any effect on metastatic colonisation potential, although all 3 enzymes were shown to be active and specific in cleaving their purified substrates, under the conditions in which they were used on the cells. Trypsin and neuraminidase were also shown to release characteristic components from the surfaces of living tumour cells, although hyaluronidase did not release detectable quantities of N-acetyl glucosamine indicating that there is little hyaluronic acid-related mucopolysaccharide on the surface of these mammary tumour cells. The results provide direct evidence suggesting that surface protein composition exerts an effect on the metastatic colonisation capability of mammary tumour cells. IMAGES: |
format | Text |
id | pubmed-2011500 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1983 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-20115002009-09-10 Effect of enzymic removal of cell surface constituents on metastatic colonisation potential of mouse mammary tumour cells. Sargent, N. S. Price, J. E. Tarin, D. Br J Cancer Research Article Trypsin treatment of viable cells from 24 spontaneous murine mammary carcinomas resulted in a mild but reproducible diminution in their capability to colonise the lung after i.v. reinoculation but did not alter the distribution of deposits formed. The effects were similar on tumours of high and of low colonisation potentials. Neuraminidase and hyaluronidase did not exert any effect on metastatic colonisation potential, although all 3 enzymes were shown to be active and specific in cleaving their purified substrates, under the conditions in which they were used on the cells. Trypsin and neuraminidase were also shown to release characteristic components from the surfaces of living tumour cells, although hyaluronidase did not release detectable quantities of N-acetyl glucosamine indicating that there is little hyaluronic acid-related mucopolysaccharide on the surface of these mammary tumour cells. The results provide direct evidence suggesting that surface protein composition exerts an effect on the metastatic colonisation capability of mammary tumour cells. IMAGES: Nature Publishing Group 1983-10 /pmc/articles/PMC2011500/ /pubmed/6626455 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Sargent, N. S. Price, J. E. Tarin, D. Effect of enzymic removal of cell surface constituents on metastatic colonisation potential of mouse mammary tumour cells. |
title | Effect of enzymic removal of cell surface constituents on metastatic colonisation potential of mouse mammary tumour cells. |
title_full | Effect of enzymic removal of cell surface constituents on metastatic colonisation potential of mouse mammary tumour cells. |
title_fullStr | Effect of enzymic removal of cell surface constituents on metastatic colonisation potential of mouse mammary tumour cells. |
title_full_unstemmed | Effect of enzymic removal of cell surface constituents on metastatic colonisation potential of mouse mammary tumour cells. |
title_short | Effect of enzymic removal of cell surface constituents on metastatic colonisation potential of mouse mammary tumour cells. |
title_sort | effect of enzymic removal of cell surface constituents on metastatic colonisation potential of mouse mammary tumour cells. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011500/ https://www.ncbi.nlm.nih.gov/pubmed/6626455 |
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