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Simultaneous micro measurement of steroid receptors in breast cancer.
The oestradiol (RE) and progesterone (RP) receptor levels were analyzed in 26 tumour fragments (200-500 mg) from breast cancer patients. After pulverization of tissue, one part was analyzed by the routine dextran-coated charcoal (DCC) method and the other by a micromethod as follows: (i) cytosol inc...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1983
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011502/ https://www.ncbi.nlm.nih.gov/pubmed/6626456 |
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author | Milano, G. Moll, J. L. Formento, J. L. Francoual, M. Krebs, B. P. Namer, M. Boublil, J. L. Lalanne, C. M. |
author_facet | Milano, G. Moll, J. L. Formento, J. L. Francoual, M. Krebs, B. P. Namer, M. Boublil, J. L. Lalanne, C. M. |
author_sort | Milano, G. |
collection | PubMed |
description | The oestradiol (RE) and progesterone (RP) receptor levels were analyzed in 26 tumour fragments (200-500 mg) from breast cancer patients. After pulverization of tissue, one part was analyzed by the routine dextran-coated charcoal (DCC) method and the other by a micromethod as follows: (i) cytosol incubation using the DCC method but in the simultaneous presence of [3H]oestradiol and [3H]R5020 (ii) extraction of the steroids bound to the receptor by precipitation with ethanol/TCA (iii) high pressure liquid chromatography (HPLC) on a modular system, with a C185 microns column and an elution by gradient mixture methanol/water. The fractions were collected and the radioactivity counted. The separation of oestradiol from R 5020 was rapid and complete. In addition dexamethasone was separated by this system making possible triple measures of RE, RP and glucocorticoid receptors. A highly significant correlation was obtained between the 2 methods: RE = 0.996, P less than 0.001; RP r = 0.975, P less than 0.001, implying that the thresholds of positivity, i.e. for therapeutic decisions, remain unchanged. Simultaneous measurement of RE and RP in a single needle biopsy is possible with this micromethod. |
format | Text |
id | pubmed-2011502 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1983 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-20115022009-09-10 Simultaneous micro measurement of steroid receptors in breast cancer. Milano, G. Moll, J. L. Formento, J. L. Francoual, M. Krebs, B. P. Namer, M. Boublil, J. L. Lalanne, C. M. Br J Cancer Research Article The oestradiol (RE) and progesterone (RP) receptor levels were analyzed in 26 tumour fragments (200-500 mg) from breast cancer patients. After pulverization of tissue, one part was analyzed by the routine dextran-coated charcoal (DCC) method and the other by a micromethod as follows: (i) cytosol incubation using the DCC method but in the simultaneous presence of [3H]oestradiol and [3H]R5020 (ii) extraction of the steroids bound to the receptor by precipitation with ethanol/TCA (iii) high pressure liquid chromatography (HPLC) on a modular system, with a C185 microns column and an elution by gradient mixture methanol/water. The fractions were collected and the radioactivity counted. The separation of oestradiol from R 5020 was rapid and complete. In addition dexamethasone was separated by this system making possible triple measures of RE, RP and glucocorticoid receptors. A highly significant correlation was obtained between the 2 methods: RE = 0.996, P less than 0.001; RP r = 0.975, P less than 0.001, implying that the thresholds of positivity, i.e. for therapeutic decisions, remain unchanged. Simultaneous measurement of RE and RP in a single needle biopsy is possible with this micromethod. Nature Publishing Group 1983-10 /pmc/articles/PMC2011502/ /pubmed/6626456 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Milano, G. Moll, J. L. Formento, J. L. Francoual, M. Krebs, B. P. Namer, M. Boublil, J. L. Lalanne, C. M. Simultaneous micro measurement of steroid receptors in breast cancer. |
title | Simultaneous micro measurement of steroid receptors in breast cancer. |
title_full | Simultaneous micro measurement of steroid receptors in breast cancer. |
title_fullStr | Simultaneous micro measurement of steroid receptors in breast cancer. |
title_full_unstemmed | Simultaneous micro measurement of steroid receptors in breast cancer. |
title_short | Simultaneous micro measurement of steroid receptors in breast cancer. |
title_sort | simultaneous micro measurement of steroid receptors in breast cancer. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011502/ https://www.ncbi.nlm.nih.gov/pubmed/6626456 |
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