Centrosomal pre-integration latency of HIV-1 in quiescent cells

Human immunodeficiency virus type 1 (HIV-1) efficiently replicates in dividing and non-dividing cells. However, HIV-1 infection is blocked at an early post-entry step in quiescent CD4+ T cells in vitro. The molecular basis of this restriction is still poorly understood. Here, we show that in quiesce...

Descripción completa

Detalles Bibliográficos
Autores principales: Zamborlini, Alessia, Lehmann-Che, Jacqueline, Clave, Emmanuel, Giron, Marie-Lou, Tobaly-Tapiero, Joëlle, Roingeard, Philippe, Emiliani, Stéphane, Toubert, Antoine, de Thé, Hugues, Saïb, Ali
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2014762/
https://www.ncbi.nlm.nih.gov/pubmed/17845727
http://dx.doi.org/10.1186/1742-4690-4-63
_version_ 1782136562373361664
author Zamborlini, Alessia
Lehmann-Che, Jacqueline
Clave, Emmanuel
Giron, Marie-Lou
Tobaly-Tapiero, Joëlle
Roingeard, Philippe
Emiliani, Stéphane
Toubert, Antoine
de Thé, Hugues
Saïb, Ali
author_facet Zamborlini, Alessia
Lehmann-Che, Jacqueline
Clave, Emmanuel
Giron, Marie-Lou
Tobaly-Tapiero, Joëlle
Roingeard, Philippe
Emiliani, Stéphane
Toubert, Antoine
de Thé, Hugues
Saïb, Ali
author_sort Zamborlini, Alessia
collection PubMed
description Human immunodeficiency virus type 1 (HIV-1) efficiently replicates in dividing and non-dividing cells. However, HIV-1 infection is blocked at an early post-entry step in quiescent CD4+ T cells in vitro. The molecular basis of this restriction is still poorly understood. Here, we show that in quiescent cells, incoming HIV-1 sub-viral complexes concentrate and stably reside at the centrosome for several weeks. Upon cell activation, viral replication resumes leading to viral gene expression. Thus, HIV-1 can persist in quiescent cells as a stable, centrosome-associated, pre-integration intermediate.
format Text
id pubmed-2014762
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-20147622007-10-11 Centrosomal pre-integration latency of HIV-1 in quiescent cells Zamborlini, Alessia Lehmann-Che, Jacqueline Clave, Emmanuel Giron, Marie-Lou Tobaly-Tapiero, Joëlle Roingeard, Philippe Emiliani, Stéphane Toubert, Antoine de Thé, Hugues Saïb, Ali Retrovirology Short Report Human immunodeficiency virus type 1 (HIV-1) efficiently replicates in dividing and non-dividing cells. However, HIV-1 infection is blocked at an early post-entry step in quiescent CD4+ T cells in vitro. The molecular basis of this restriction is still poorly understood. Here, we show that in quiescent cells, incoming HIV-1 sub-viral complexes concentrate and stably reside at the centrosome for several weeks. Upon cell activation, viral replication resumes leading to viral gene expression. Thus, HIV-1 can persist in quiescent cells as a stable, centrosome-associated, pre-integration intermediate. BioMed Central 2007-09-10 /pmc/articles/PMC2014762/ /pubmed/17845727 http://dx.doi.org/10.1186/1742-4690-4-63 Text en Copyright © 2007 Zamborlini et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Zamborlini, Alessia
Lehmann-Che, Jacqueline
Clave, Emmanuel
Giron, Marie-Lou
Tobaly-Tapiero, Joëlle
Roingeard, Philippe
Emiliani, Stéphane
Toubert, Antoine
de Thé, Hugues
Saïb, Ali
Centrosomal pre-integration latency of HIV-1 in quiescent cells
title Centrosomal pre-integration latency of HIV-1 in quiescent cells
title_full Centrosomal pre-integration latency of HIV-1 in quiescent cells
title_fullStr Centrosomal pre-integration latency of HIV-1 in quiescent cells
title_full_unstemmed Centrosomal pre-integration latency of HIV-1 in quiescent cells
title_short Centrosomal pre-integration latency of HIV-1 in quiescent cells
title_sort centrosomal pre-integration latency of hiv-1 in quiescent cells
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2014762/
https://www.ncbi.nlm.nih.gov/pubmed/17845727
http://dx.doi.org/10.1186/1742-4690-4-63
work_keys_str_mv AT zamborlinialessia centrosomalpreintegrationlatencyofhiv1inquiescentcells
AT lehmannchejacqueline centrosomalpreintegrationlatencyofhiv1inquiescentcells
AT claveemmanuel centrosomalpreintegrationlatencyofhiv1inquiescentcells
AT gironmarielou centrosomalpreintegrationlatencyofhiv1inquiescentcells
AT tobalytapierojoelle centrosomalpreintegrationlatencyofhiv1inquiescentcells
AT roingeardphilippe centrosomalpreintegrationlatencyofhiv1inquiescentcells
AT emilianistephane centrosomalpreintegrationlatencyofhiv1inquiescentcells
AT toubertantoine centrosomalpreintegrationlatencyofhiv1inquiescentcells
AT dethehugues centrosomalpreintegrationlatencyofhiv1inquiescentcells
AT saibali centrosomalpreintegrationlatencyofhiv1inquiescentcells

Ejemplares similares