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One step construction of PCR mutagenized libraries for genetic analysis by recombination cloning
Recombination cloning encompasses a set of technologies that transfer gene sequences between vectors through site-specific recombination. Due in part to the instability of linear DNA in bacteria, both the initial capture and subsequent transfer of gene sequences is often performed using purified rec...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2018627/ https://www.ncbi.nlm.nih.gov/pubmed/17702758 http://dx.doi.org/10.1093/nar/gkm583 |