Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR

Real-time PCR assays have recently been developed for diagnostic and research purposes. Signal generation in real-time PCR is achieved with probe designs that usually depend on exonuclease activity of DNA polymerase (e.g. TaqMan probe) or oligonucleotide hybridization (e.g. molecular beacon). Probe...

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Detalles Bibliográficos
Autores principales: Huang, Shihai, Salituro, John, Tang, Ning, Luk, Ka-Cheung, Hackett, John, Swanson, Priscilla, Cloherty, Gavin, Mak, Wai-Bing, Robinson, John, Abravaya, Klara
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2018630/
https://www.ncbi.nlm.nih.gov/pubmed/17693434
http://dx.doi.org/10.1093/nar/gkm551

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