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Fluorescent probing for RNA molecules by an unnatural base-pair system
Fluorescent labeling of nucleic acids is widely used in basic research and medical applications. We describe the efficient site-specific incorporation of a fluorescent base analog, 2-amino-6-(2-thienyl)purine (s), into RNA by transcription mediated by an unnatural base pair between s and pyrrole-2-c...
Autores principales: | Kimoto, Michiko, Mitsui, Tsuneo, Harada, Yoko, Sato, Akira, Yokoyama, Shigeyuki, Hirao, Ichiro |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2018647/ https://www.ncbi.nlm.nih.gov/pubmed/17693436 http://dx.doi.org/10.1093/nar/gkm508 |
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