The persistence of unmetabolized 3H-7,12-dimethylbenz(a)anthracene in regenerating rat liver.

The hepatic subcellular distribution, binding and persistence of 3H-7,12-dimethylbenz(a)anthracene were compared in partially hepatectomized rats and in intact controls. By 2 weeks after injection, intact liver homogenates contained only 9% of the total radioactivity present 4 h after injection; reg...

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Detalles Bibliográficos
Autores principales: Tomsak, R. L., Cook, R. T.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1975
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2024757/
https://www.ncbi.nlm.nih.gov/pubmed/813754
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author Tomsak, R. L.
Cook, R. T.
author_facet Tomsak, R. L.
Cook, R. T.
author_sort Tomsak, R. L.
collection PubMed
description The hepatic subcellular distribution, binding and persistence of 3H-7,12-dimethylbenz(a)anthracene were compared in partially hepatectomized rats and in intact controls. By 2 weeks after injection, intact liver homogenates contained only 9% of the total radioactivity present 4 h after injection; regenerated liver contained 60% in spite of a tripling in liver mass during this time. Cell fractions isolated from regenerated liver had 9-59 fold greater hexane extractable specific activities than those from intact liver. The radioactivity present in hexane extracts co-chromatographed with a 3H-7,12-dimethylbenz(a)anthracene standard. Preliminary experiments demonstrated that liver microsomes isolated from DMBA treated partially hepatectomized animals metabolized less DMBA in vitro than did microsomes isolated from DMBA treated intact animals. The greater persistence of unmetabolized DMBA may be related to the greater carcinogenicity of this compound for regenerating, as compared with intact, rat liver.
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spelling pubmed-20247572009-09-10 The persistence of unmetabolized 3H-7,12-dimethylbenz(a)anthracene in regenerating rat liver. Tomsak, R. L. Cook, R. T. Br J Cancer Research Article The hepatic subcellular distribution, binding and persistence of 3H-7,12-dimethylbenz(a)anthracene were compared in partially hepatectomized rats and in intact controls. By 2 weeks after injection, intact liver homogenates contained only 9% of the total radioactivity present 4 h after injection; regenerated liver contained 60% in spite of a tripling in liver mass during this time. Cell fractions isolated from regenerated liver had 9-59 fold greater hexane extractable specific activities than those from intact liver. The radioactivity present in hexane extracts co-chromatographed with a 3H-7,12-dimethylbenz(a)anthracene standard. Preliminary experiments demonstrated that liver microsomes isolated from DMBA treated partially hepatectomized animals metabolized less DMBA in vitro than did microsomes isolated from DMBA treated intact animals. The greater persistence of unmetabolized DMBA may be related to the greater carcinogenicity of this compound for regenerating, as compared with intact, rat liver. Nature Publishing Group 1975-10 /pmc/articles/PMC2024757/ /pubmed/813754 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Tomsak, R. L.
Cook, R. T.
The persistence of unmetabolized 3H-7,12-dimethylbenz(a)anthracene in regenerating rat liver.
title The persistence of unmetabolized 3H-7,12-dimethylbenz(a)anthracene in regenerating rat liver.
title_full The persistence of unmetabolized 3H-7,12-dimethylbenz(a)anthracene in regenerating rat liver.
title_fullStr The persistence of unmetabolized 3H-7,12-dimethylbenz(a)anthracene in regenerating rat liver.
title_full_unstemmed The persistence of unmetabolized 3H-7,12-dimethylbenz(a)anthracene in regenerating rat liver.
title_short The persistence of unmetabolized 3H-7,12-dimethylbenz(a)anthracene in regenerating rat liver.
title_sort persistence of unmetabolized 3h-7,12-dimethylbenz(a)anthracene in regenerating rat liver.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2024757/
https://www.ncbi.nlm.nih.gov/pubmed/813754
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