Smooth muscle associated antigen in astrocytes and astrocytomata.

Four human and 4 rat astrocytomata and mammalian adult brain and spinal cord were examined by indirect immunofluorescence with human serum containing smooth muscle antibody. Cryostat sections of astrocytomata showed staining of the tumour cell cytoplasm and processes while in normal adult brain and...

Descripción completa

Detalles Bibliográficos
Autores principales: Toh, B. H., Muller, H. K., Elrick, W. L.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1976
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2024934/
https://www.ncbi.nlm.nih.gov/pubmed/769816
_version_ 1782136661754249216
author Toh, B. H.
Muller, H. K.
Elrick, W. L.
author_facet Toh, B. H.
Muller, H. K.
Elrick, W. L.
author_sort Toh, B. H.
collection PubMed
description Four human and 4 rat astrocytomata and mammalian adult brain and spinal cord were examined by indirect immunofluorescence with human serum containing smooth muscle antibody. Cryostat sections of astrocytomata showed staining of the tumour cell cytoplasm and processes while in normal adult brain and spinal cord the entire astrocyte stained. Impression film and tissue culture monolayers of astrocytomata showed staining of cell processes and a fine, filamentous network in the cell body. The reaction with astrocytoma tumour cells was stronger than that with the corresponding normal astrocytes. Specificity of the staining reaction was established by its prevention on neutralization absorptions of the serum with extracts or homogeneous of smooth muscle. The presence of smooth muscle-associated antigen in astrocytes and astrocytomata is indicative of contractile protein providing a mechanism of cell movement in vivo. IMAGES:
format Text
id pubmed-2024934
institution National Center for Biotechnology Information
language English
publishDate 1976
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-20249342009-09-10 Smooth muscle associated antigen in astrocytes and astrocytomata. Toh, B. H. Muller, H. K. Elrick, W. L. Br J Cancer Research Article Four human and 4 rat astrocytomata and mammalian adult brain and spinal cord were examined by indirect immunofluorescence with human serum containing smooth muscle antibody. Cryostat sections of astrocytomata showed staining of the tumour cell cytoplasm and processes while in normal adult brain and spinal cord the entire astrocyte stained. Impression film and tissue culture monolayers of astrocytomata showed staining of cell processes and a fine, filamentous network in the cell body. The reaction with astrocytoma tumour cells was stronger than that with the corresponding normal astrocytes. Specificity of the staining reaction was established by its prevention on neutralization absorptions of the serum with extracts or homogeneous of smooth muscle. The presence of smooth muscle-associated antigen in astrocytes and astrocytomata is indicative of contractile protein providing a mechanism of cell movement in vivo. IMAGES: Nature Publishing Group 1976-02 /pmc/articles/PMC2024934/ /pubmed/769816 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Toh, B. H.
Muller, H. K.
Elrick, W. L.
Smooth muscle associated antigen in astrocytes and astrocytomata.
title Smooth muscle associated antigen in astrocytes and astrocytomata.
title_full Smooth muscle associated antigen in astrocytes and astrocytomata.
title_fullStr Smooth muscle associated antigen in astrocytes and astrocytomata.
title_full_unstemmed Smooth muscle associated antigen in astrocytes and astrocytomata.
title_short Smooth muscle associated antigen in astrocytes and astrocytomata.
title_sort smooth muscle associated antigen in astrocytes and astrocytomata.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2024934/
https://www.ncbi.nlm.nih.gov/pubmed/769816
work_keys_str_mv AT tohbh smoothmuscleassociatedantigeninastrocytesandastrocytomata
AT mullerhk smoothmuscleassociatedantigeninastrocytesandastrocytomata
AT elrickwl smoothmuscleassociatedantigeninastrocytesandastrocytomata

Ejemplares similares