The effect of aflatoxins on the incorporation of RNA and protein precursors by isolated hepatocytes.

Hepatocytes prepared by a simplified enzymatic technique were active in the incorporation of RNA and protein precursors into acid-insoluble material. The incorporation of RNA precursors was very markedly inhibited by low levels of aflatoxin B1 and G1 but not by aflatoxins B2 and G2. The activity of...

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Detalles Bibliográficos
Autores principales: McIntosh, P. R., Evans, I. H., Rabin, B. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1976
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025075/
https://www.ncbi.nlm.nih.gov/pubmed/5101
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author McIntosh, P. R.
Evans, I. H.
Rabin, B. R.
author_facet McIntosh, P. R.
Evans, I. H.
Rabin, B. R.
author_sort McIntosh, P. R.
collection PubMed
description Hepatocytes prepared by a simplified enzymatic technique were active in the incorporation of RNA and protein precursors into acid-insoluble material. The incorporation of RNA precursors was very markedly inhibited by low levels of aflatoxin B1 and G1 but not by aflatoxins B2 and G2. The activity of mixed function oxidases (MFO), the drug-metabolizing system of the endoplasmic reticulum, could be suppressed in these cells by SKF525A or stimulated by NADPH. SKF525A caused a reduction in the inhibition by aflatoxin B1 of the incorporation of RNA precursor into macromolecules. This finding suggests that a metabolite of aflatoxin B1 is the actual inhibitor of RNA synthesis in the cells. Measurement of lactate dehydrogenase activity showed these cells to be leaky on incubation at 37 degrees C and thus not suitable for studies of protein secretion.
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spelling pubmed-20250752009-09-10 The effect of aflatoxins on the incorporation of RNA and protein precursors by isolated hepatocytes. McIntosh, P. R. Evans, I. H. Rabin, B. R. Br J Cancer Research Article Hepatocytes prepared by a simplified enzymatic technique were active in the incorporation of RNA and protein precursors into acid-insoluble material. The incorporation of RNA precursors was very markedly inhibited by low levels of aflatoxin B1 and G1 but not by aflatoxins B2 and G2. The activity of mixed function oxidases (MFO), the drug-metabolizing system of the endoplasmic reticulum, could be suppressed in these cells by SKF525A or stimulated by NADPH. SKF525A caused a reduction in the inhibition by aflatoxin B1 of the incorporation of RNA precursor into macromolecules. This finding suggests that a metabolite of aflatoxin B1 is the actual inhibitor of RNA synthesis in the cells. Measurement of lactate dehydrogenase activity showed these cells to be leaky on incubation at 37 degrees C and thus not suitable for studies of protein secretion. Nature Publishing Group 1976-04 /pmc/articles/PMC2025075/ /pubmed/5101 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
McIntosh, P. R.
Evans, I. H.
Rabin, B. R.
The effect of aflatoxins on the incorporation of RNA and protein precursors by isolated hepatocytes.
title The effect of aflatoxins on the incorporation of RNA and protein precursors by isolated hepatocytes.
title_full The effect of aflatoxins on the incorporation of RNA and protein precursors by isolated hepatocytes.
title_fullStr The effect of aflatoxins on the incorporation of RNA and protein precursors by isolated hepatocytes.
title_full_unstemmed The effect of aflatoxins on the incorporation of RNA and protein precursors by isolated hepatocytes.
title_short The effect of aflatoxins on the incorporation of RNA and protein precursors by isolated hepatocytes.
title_sort effect of aflatoxins on the incorporation of rna and protein precursors by isolated hepatocytes.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025075/
https://www.ncbi.nlm.nih.gov/pubmed/5101
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