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Chemical transformation of Chinese hamster cells: II. Appearance of marker chromosomes in transformed cells.
The chromosomes of 12 samples of cultured Chinese hamster kidney and prostate cells (4 normal and 8 transformed), whose tissue culture properties have already been described (Kirkland, 1976) have been examined for numerical change and for the appearance of abnormal markers. Six transformed kidney su...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1976
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025144/ https://www.ncbi.nlm.nih.gov/pubmed/986826 |
Sumario: | The chromosomes of 12 samples of cultured Chinese hamster kidney and prostate cells (4 normal and 8 transformed), whose tissue culture properties have already been described (Kirkland, 1976) have been examined for numerical change and for the appearance of abnormal markers. Six transformed kidney subclones contained a consistent telocentric marker not present in the normal parental cell, and Giemsa banding demonstrated this to be the centromere and the long (q) arm of the number 4 chromosome in all cases. Two transformed prostate subclones also contained a consistent telocentric marker, not present in similarly derived normal subclones or in the normal parental cell, and Giemsa banding demonstrated this to be a different fragment (the centromere and most of the p arm) of the number 4 chromosome. It is believed that the use of a mixed-serum culture medium, designed to stabilize the karyotype of cultured Chinese hamster cells, is at least partly responsible for the detection of these transformation-associated chromosome changes. IMAGES: |
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