A colony-forming assay for human tumour xenografts using agar in diffusion chambers.

A technique for growing colonies from single-cell suspensions of human tumour xenografts using agar in diffusion chambers is described. Modified Millipore diffusion chambers containing tumour cells in semi-solid agar-medium were implanted into the peritoneal cavity of pre-irradiated mice and provide...

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Detalles Bibliográficos
Autores principales: Smith, I. E., Courtenay, V. D., Gordon, M. Y.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1976
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025199/
https://www.ncbi.nlm.nih.gov/pubmed/999782
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author Smith, I. E.
Courtenay, V. D.
Gordon, M. Y.
author_facet Smith, I. E.
Courtenay, V. D.
Gordon, M. Y.
author_sort Smith, I. E.
collection PubMed
description A technique for growing colonies from single-cell suspensions of human tumour xenografts using agar in diffusion chambers is described. Modified Millipore diffusion chambers containing tumour cells in semi-solid agar-medium were implanted into the peritoneal cavity of pre-irradiated mice and provided standard culture conditions for the study of colony-forming cells. All 11 xenograft tumours so far studied produced colonies. The incubation period for colony growth ranged from 12 to 28 days and the plating efficiency ranged from 0-3% to 16% for different tumours, but both parameters were constant for each individual tumour. The reproducibility of the system provides a colony-forming assay which can be used to study the effects of irradiation and cytotoxic drugs on human tumour clonogenic cells and may therefore have some advantages over similar assays based on experimental animal tumours. IMAGES:
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spelling pubmed-20251992009-09-10 A colony-forming assay for human tumour xenografts using agar in diffusion chambers. Smith, I. E. Courtenay, V. D. Gordon, M. Y. Br J Cancer Research Article A technique for growing colonies from single-cell suspensions of human tumour xenografts using agar in diffusion chambers is described. Modified Millipore diffusion chambers containing tumour cells in semi-solid agar-medium were implanted into the peritoneal cavity of pre-irradiated mice and provided standard culture conditions for the study of colony-forming cells. All 11 xenograft tumours so far studied produced colonies. The incubation period for colony growth ranged from 12 to 28 days and the plating efficiency ranged from 0-3% to 16% for different tumours, but both parameters were constant for each individual tumour. The reproducibility of the system provides a colony-forming assay which can be used to study the effects of irradiation and cytotoxic drugs on human tumour clonogenic cells and may therefore have some advantages over similar assays based on experimental animal tumours. IMAGES: Nature Publishing Group 1976-11 /pmc/articles/PMC2025199/ /pubmed/999782 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Smith, I. E.
Courtenay, V. D.
Gordon, M. Y.
A colony-forming assay for human tumour xenografts using agar in diffusion chambers.
title A colony-forming assay for human tumour xenografts using agar in diffusion chambers.
title_full A colony-forming assay for human tumour xenografts using agar in diffusion chambers.
title_fullStr A colony-forming assay for human tumour xenografts using agar in diffusion chambers.
title_full_unstemmed A colony-forming assay for human tumour xenografts using agar in diffusion chambers.
title_short A colony-forming assay for human tumour xenografts using agar in diffusion chambers.
title_sort colony-forming assay for human tumour xenografts using agar in diffusion chambers.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025199/
https://www.ncbi.nlm.nih.gov/pubmed/999782
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