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Stimulation of autologous blood lymphocytes by malignant lymphoma cells and homogenates.
The blastogenic response to autologous blood lymphocytes to whole-cell suspensions and to homogenates obtained from malignant lymphoma tissue has been investigated. Spleens were obtained from patients in whom laparotomy was performed for staging of malignant lymphoma. Cell suspensions prepared from...
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1976
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025238/ https://www.ncbi.nlm.nih.gov/pubmed/1008989 |
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author | Ludgate, M. E. Gough, J. |
author_facet | Ludgate, M. E. Gough, J. |
author_sort | Ludgate, M. E. |
collection | PubMed |
description | The blastogenic response to autologous blood lymphocytes to whole-cell suspensions and to homogenates obtained from malignant lymphoma tissue has been investigated. Spleens were obtained from patients in whom laparotomy was performed for staging of malignant lymphoma. Cell suspensions prepared from tumour nodules were treated with mitomycin C and allowed to react with separated autologous blood lymphocytes for 6 days. Lymphocyte stimulation was measured by liquid scintillation counting after exposure to 3H-TdR. Cultures were also prepared in which autologous lymphocytes were treated with spleen tumour homogenate. Control experiments used spleens from staging procedures in which no tumour deposits were present, and normal spleens removed incidentally during other operations. In the controls, the uptake of TdR was never more than twice that of unstimulated lymphocytes. Greater degrees of lymphocyte stimulation were seen in 6 out of 14 patients, using whole tumour cells, and in 7 out of 16 patients, using tumour homogenates. The results indicate an antigenic difference between tumour and host cells, and suggest that lymphocytes can react to a tumour-associated antigen. |
format | Text |
id | pubmed-2025238 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1976 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-20252382009-09-10 Stimulation of autologous blood lymphocytes by malignant lymphoma cells and homogenates. Ludgate, M. E. Gough, J. Br J Cancer Research Article The blastogenic response to autologous blood lymphocytes to whole-cell suspensions and to homogenates obtained from malignant lymphoma tissue has been investigated. Spleens were obtained from patients in whom laparotomy was performed for staging of malignant lymphoma. Cell suspensions prepared from tumour nodules were treated with mitomycin C and allowed to react with separated autologous blood lymphocytes for 6 days. Lymphocyte stimulation was measured by liquid scintillation counting after exposure to 3H-TdR. Cultures were also prepared in which autologous lymphocytes were treated with spleen tumour homogenate. Control experiments used spleens from staging procedures in which no tumour deposits were present, and normal spleens removed incidentally during other operations. In the controls, the uptake of TdR was never more than twice that of unstimulated lymphocytes. Greater degrees of lymphocyte stimulation were seen in 6 out of 14 patients, using whole tumour cells, and in 7 out of 16 patients, using tumour homogenates. The results indicate an antigenic difference between tumour and host cells, and suggest that lymphocytes can react to a tumour-associated antigen. Nature Publishing Group 1976-12 /pmc/articles/PMC2025238/ /pubmed/1008989 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Ludgate, M. E. Gough, J. Stimulation of autologous blood lymphocytes by malignant lymphoma cells and homogenates. |
title | Stimulation of autologous blood lymphocytes by malignant lymphoma cells and homogenates. |
title_full | Stimulation of autologous blood lymphocytes by malignant lymphoma cells and homogenates. |
title_fullStr | Stimulation of autologous blood lymphocytes by malignant lymphoma cells and homogenates. |
title_full_unstemmed | Stimulation of autologous blood lymphocytes by malignant lymphoma cells and homogenates. |
title_short | Stimulation of autologous blood lymphocytes by malignant lymphoma cells and homogenates. |
title_sort | stimulation of autologous blood lymphocytes by malignant lymphoma cells and homogenates. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025238/ https://www.ncbi.nlm.nih.gov/pubmed/1008989 |
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