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Avascular and vascular phases of tumour growth in the chick embryo.
The chick embryo was used to study the relationship between the onset of tumour neovascularization and tumour growth. Walker 256 carcinosarcoma was implanted on the chrioallantoic membrane (CAM) of about 600 embryos aged 5-16 days. Tumour diameter and changes in the CAM vasculature in response to th...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1977
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025276/ https://www.ncbi.nlm.nih.gov/pubmed/856240 |
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author | Knighton, D. Ausprunk, D. Tapper, D. Folkman, J. |
author_facet | Knighton, D. Ausprunk, D. Tapper, D. Folkman, J. |
author_sort | Knighton, D. |
collection | PubMed |
description | The chick embryo was used to study the relationship between the onset of tumour neovascularization and tumour growth. Walker 256 carcinosarcoma was implanted on the chrioallantoic membrane (CAM) of about 600 embryos aged 5-16 days. Tumour diameter and changes in the CAM vasculature in response to the implants were recorded daily. Representative tumours were examined by light microscopy of Epon-embedded tissue and autoradiography after injection of [3H]-thymidine. Tumours remained avascular for 72 h, after which they were penetrated by new blood vessels and began a phase of rapid growth. The rate of growth during this vascular phase was greatest for implants on 5- and 6-day-old embryos and decreased the later the day of implantation. The time of onset of tumour angiogenesis appears to be independent of the immunological state of the chick embryo, although the rate of growth after vascularization may be modified by the onset of immunity. This study suggests that the avascular and vascular phases of tumour growth are separable, and that the avascular tumour population lives under the growth constraints which limit the size of a tumour spheroid growing in soft agar or aqueous humour. IMAGES: |
format | Text |
id | pubmed-2025276 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1977 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-20252762009-09-10 Avascular and vascular phases of tumour growth in the chick embryo. Knighton, D. Ausprunk, D. Tapper, D. Folkman, J. Br J Cancer Research Article The chick embryo was used to study the relationship between the onset of tumour neovascularization and tumour growth. Walker 256 carcinosarcoma was implanted on the chrioallantoic membrane (CAM) of about 600 embryos aged 5-16 days. Tumour diameter and changes in the CAM vasculature in response to the implants were recorded daily. Representative tumours were examined by light microscopy of Epon-embedded tissue and autoradiography after injection of [3H]-thymidine. Tumours remained avascular for 72 h, after which they were penetrated by new blood vessels and began a phase of rapid growth. The rate of growth during this vascular phase was greatest for implants on 5- and 6-day-old embryos and decreased the later the day of implantation. The time of onset of tumour angiogenesis appears to be independent of the immunological state of the chick embryo, although the rate of growth after vascularization may be modified by the onset of immunity. This study suggests that the avascular and vascular phases of tumour growth are separable, and that the avascular tumour population lives under the growth constraints which limit the size of a tumour spheroid growing in soft agar or aqueous humour. IMAGES: Nature Publishing Group 1977-03 /pmc/articles/PMC2025276/ /pubmed/856240 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Knighton, D. Ausprunk, D. Tapper, D. Folkman, J. Avascular and vascular phases of tumour growth in the chick embryo. |
title | Avascular and vascular phases of tumour growth in the chick embryo. |
title_full | Avascular and vascular phases of tumour growth in the chick embryo. |
title_fullStr | Avascular and vascular phases of tumour growth in the chick embryo. |
title_full_unstemmed | Avascular and vascular phases of tumour growth in the chick embryo. |
title_short | Avascular and vascular phases of tumour growth in the chick embryo. |
title_sort | avascular and vascular phases of tumour growth in the chick embryo. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025276/ https://www.ncbi.nlm.nih.gov/pubmed/856240 |
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