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Radiolabelling of Corynebacterium parvum and its distribution in mice.
Corynebacterium parvum was labelled by growing live bacteria in the presence of [3H]thymidine. The bacteria were killed by formalin, washed thoroughly and resuspended at a concentration of 7 mg dry weight/ml. An activity of 1-6 X 10(5) ct/min/0-1 ml was obtained. The biological properties (inhibitio...
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1977
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025284/ https://www.ncbi.nlm.nih.gov/pubmed/851511 |
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author | Sadler, T. E. Cramp, W. A. Castro, J. E. |
author_facet | Sadler, T. E. Cramp, W. A. Castro, J. E. |
author_sort | Sadler, T. E. |
collection | PubMed |
description | Corynebacterium parvum was labelled by growing live bacteria in the presence of [3H]thymidine. The bacteria were killed by formalin, washed thoroughly and resuspended at a concentration of 7 mg dry weight/ml. An activity of 1-6 X 10(5) ct/min/0-1 ml was obtained. The biological properties (inhibition of tumour growth and hepatosplenomegaly) of the labelled C. parvum were compared with those of commercially available vaccine, and were found to be similar. Labelled C. parvum was injected i.v., i.p., or s.c. into normal C57BL mice and the localization of activity determined at 4 h and 1,3,7 and 14 days after injection. After i.v. or i.p. injection, highest counts were recorded in the liver. Moderate activity was found in the spleen, lungs and small gut. After s.c. injection, the majority of radioactive label was detected at the site of injection and little found in other tissues. The distribution of injected C. parvum was also studied in mice bearing Lewis tumour, and was found to be similar to that in normal mice. Moderate amounts of labelled C. parvum were recovered from tumour. There appeared to be no relationship between the antitumour effect of C. parvum given by a particular route of injection and the concentration of C. parvum recovered from the tumour. IMAGES: |
format | Text |
id | pubmed-2025284 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1977 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-20252842009-09-10 Radiolabelling of Corynebacterium parvum and its distribution in mice. Sadler, T. E. Cramp, W. A. Castro, J. E. Br J Cancer Research Article Corynebacterium parvum was labelled by growing live bacteria in the presence of [3H]thymidine. The bacteria were killed by formalin, washed thoroughly and resuspended at a concentration of 7 mg dry weight/ml. An activity of 1-6 X 10(5) ct/min/0-1 ml was obtained. The biological properties (inhibition of tumour growth and hepatosplenomegaly) of the labelled C. parvum were compared with those of commercially available vaccine, and were found to be similar. Labelled C. parvum was injected i.v., i.p., or s.c. into normal C57BL mice and the localization of activity determined at 4 h and 1,3,7 and 14 days after injection. After i.v. or i.p. injection, highest counts were recorded in the liver. Moderate activity was found in the spleen, lungs and small gut. After s.c. injection, the majority of radioactive label was detected at the site of injection and little found in other tissues. The distribution of injected C. parvum was also studied in mice bearing Lewis tumour, and was found to be similar to that in normal mice. Moderate amounts of labelled C. parvum were recovered from tumour. There appeared to be no relationship between the antitumour effect of C. parvum given by a particular route of injection and the concentration of C. parvum recovered from the tumour. IMAGES: Nature Publishing Group 1977-03 /pmc/articles/PMC2025284/ /pubmed/851511 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Sadler, T. E. Cramp, W. A. Castro, J. E. Radiolabelling of Corynebacterium parvum and its distribution in mice. |
title | Radiolabelling of Corynebacterium parvum and its distribution in mice. |
title_full | Radiolabelling of Corynebacterium parvum and its distribution in mice. |
title_fullStr | Radiolabelling of Corynebacterium parvum and its distribution in mice. |
title_full_unstemmed | Radiolabelling of Corynebacterium parvum and its distribution in mice. |
title_short | Radiolabelling of Corynebacterium parvum and its distribution in mice. |
title_sort | radiolabelling of corynebacterium parvum and its distribution in mice. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025284/ https://www.ncbi.nlm.nih.gov/pubmed/851511 |
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