Fractional incorporation of [3H]thymidine and DNA specific activity as assays of inhibition of tumour growth.

The Fractional Incorporation (FI) of [3H] thymidine ([3H]TdR) has been examined in small lung tumours after cyclophosphamide (CY) treatment in vivo and compared to the DNA specific activity (SA) at different times after treatment. FI was found to correlate with the incidence of labelled cells after...

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Detalles Bibliográficos
Autores principales: Houghton, P. J., Taylor, D. M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1977
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025308/
https://www.ncbi.nlm.nih.gov/pubmed/831761
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author Houghton, P. J.
Taylor, D. M.
author_facet Houghton, P. J.
Taylor, D. M.
author_sort Houghton, P. J.
collection PubMed
description The Fractional Incorporation (FI) of [3H] thymidine ([3H]TdR) has been examined in small lung tumours after cyclophosphamide (CY) treatment in vivo and compared to the DNA specific activity (SA) at different times after treatment. FI was found to correlate with the incidence of labelled cells after treatment, whereas SA did not, due to the loss of DNA from drug-killed cells 72 h after treatment. The FI is independent of the precursor concentration in the tissue, and therefore may give a better index of DNA synthesis in irregularly perfused tissues than SA. Following either CY or 60Co radiation treatment, the time necessary for FI to reach the pretreatment level is quite similar to the growth delay measured for the FI depression 45 h after treatment and growth delay has been established in the Lewis lung tumour, which would allow the prediction of growth delay induced by another agent to be made within 2 days of treatment. IMAGES:
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spelling pubmed-20253082009-09-10 Fractional incorporation of [3H]thymidine and DNA specific activity as assays of inhibition of tumour growth. Houghton, P. J. Taylor, D. M. Br J Cancer Research Article The Fractional Incorporation (FI) of [3H] thymidine ([3H]TdR) has been examined in small lung tumours after cyclophosphamide (CY) treatment in vivo and compared to the DNA specific activity (SA) at different times after treatment. FI was found to correlate with the incidence of labelled cells after treatment, whereas SA did not, due to the loss of DNA from drug-killed cells 72 h after treatment. The FI is independent of the precursor concentration in the tissue, and therefore may give a better index of DNA synthesis in irregularly perfused tissues than SA. Following either CY or 60Co radiation treatment, the time necessary for FI to reach the pretreatment level is quite similar to the growth delay measured for the FI depression 45 h after treatment and growth delay has been established in the Lewis lung tumour, which would allow the prediction of growth delay induced by another agent to be made within 2 days of treatment. IMAGES: Nature Publishing Group 1977-01 /pmc/articles/PMC2025308/ /pubmed/831761 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Houghton, P. J.
Taylor, D. M.
Fractional incorporation of [3H]thymidine and DNA specific activity as assays of inhibition of tumour growth.
title Fractional incorporation of [3H]thymidine and DNA specific activity as assays of inhibition of tumour growth.
title_full Fractional incorporation of [3H]thymidine and DNA specific activity as assays of inhibition of tumour growth.
title_fullStr Fractional incorporation of [3H]thymidine and DNA specific activity as assays of inhibition of tumour growth.
title_full_unstemmed Fractional incorporation of [3H]thymidine and DNA specific activity as assays of inhibition of tumour growth.
title_short Fractional incorporation of [3H]thymidine and DNA specific activity as assays of inhibition of tumour growth.
title_sort fractional incorporation of [3h]thymidine and dna specific activity as assays of inhibition of tumour growth.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025308/
https://www.ncbi.nlm.nih.gov/pubmed/831761
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