Effects of dexamethasone and betamethasone on in vitro cultures from human astrocytoma.

Cultures of human astrocytoma have been derived by collagenase digestion and are presumed, from their aneuploid karyotypes, to be predominantly neoplastic. Early passage cultures in proliferative phase have been cloned in the presence of dexamethasone and betamethasone, both commonly used in managem...

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Autores principales: Guner, M., Freshney, R. I., Morgan, D., Freshney, M. G., Thomas, D. G., Graham, D. I.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1977
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025347/
https://www.ncbi.nlm.nih.gov/pubmed/869982
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author Guner, M.
Freshney, R. I.
Morgan, D.
Freshney, M. G.
Thomas, D. G.
Graham, D. I.
author_facet Guner, M.
Freshney, R. I.
Morgan, D.
Freshney, M. G.
Thomas, D. G.
Graham, D. I.
author_sort Guner, M.
collection PubMed
description Cultures of human astrocytoma have been derived by collagenase digestion and are presumed, from their aneuploid karyotypes, to be predominantly neoplastic. Early passage cultures in proliferative phase have been cloned in the presence of dexamethasone and betamethasone, both commonly used in management of patients with brain tumours. These steroids raise both the cloning efficiency and the proliferative capacity of cells within each clone. Inhibition was detected only in very high steroid concentrations (25-50 microng/ml). Since these concentrations are unlikely to be attained in vivo it is concluded that anticipated physiological levels of these steroids enhance cell survival at low densities in culture. The significance of this in vivo is discussed. IMAGES:
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spelling pubmed-20253472009-09-10 Effects of dexamethasone and betamethasone on in vitro cultures from human astrocytoma. Guner, M. Freshney, R. I. Morgan, D. Freshney, M. G. Thomas, D. G. Graham, D. I. Br J Cancer Research Article Cultures of human astrocytoma have been derived by collagenase digestion and are presumed, from their aneuploid karyotypes, to be predominantly neoplastic. Early passage cultures in proliferative phase have been cloned in the presence of dexamethasone and betamethasone, both commonly used in management of patients with brain tumours. These steroids raise both the cloning efficiency and the proliferative capacity of cells within each clone. Inhibition was detected only in very high steroid concentrations (25-50 microng/ml). Since these concentrations are unlikely to be attained in vivo it is concluded that anticipated physiological levels of these steroids enhance cell survival at low densities in culture. The significance of this in vivo is discussed. IMAGES: Nature Publishing Group 1977-04 /pmc/articles/PMC2025347/ /pubmed/869982 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Guner, M.
Freshney, R. I.
Morgan, D.
Freshney, M. G.
Thomas, D. G.
Graham, D. I.
Effects of dexamethasone and betamethasone on in vitro cultures from human astrocytoma.
title Effects of dexamethasone and betamethasone on in vitro cultures from human astrocytoma.
title_full Effects of dexamethasone and betamethasone on in vitro cultures from human astrocytoma.
title_fullStr Effects of dexamethasone and betamethasone on in vitro cultures from human astrocytoma.
title_full_unstemmed Effects of dexamethasone and betamethasone on in vitro cultures from human astrocytoma.
title_short Effects of dexamethasone and betamethasone on in vitro cultures from human astrocytoma.
title_sort effects of dexamethasone and betamethasone on in vitro cultures from human astrocytoma.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025347/
https://www.ncbi.nlm.nih.gov/pubmed/869982
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