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Assessment of drug sensitivity of human leukaemic myeloblasts. II. The toxic effects of cytosine arabinoside on 125IUdR-labelled human leukaemic myeloblasts in mice.
Leukaemia cells from the peripheral blood and bone marrow of patients with acute myeloblastic leukaemia were labelled in vitro with [125I]5-iodo-2'-deoxy-uridine (IUdR). The myeloblasts were then injected into groups of mice and the survival of these cells estimated by measuring isotope loss, u...
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1977
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025424/ https://www.ncbi.nlm.nih.gov/pubmed/270373 |
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author | Sonis, S. T. Falcão, R. MacLennan, I. C. |
author_facet | Sonis, S. T. Falcão, R. MacLennan, I. C. |
author_sort | Sonis, S. T. |
collection | PubMed |
description | Leukaemia cells from the peripheral blood and bone marrow of patients with acute myeloblastic leukaemia were labelled in vitro with [125I]5-iodo-2'-deoxy-uridine (IUdR). The myeloblasts were then injected into groups of mice and the survival of these cells estimated by measuring isotope loss, using whole-body counting. The isotope excretion from mice treated with various doses of cytosine arabinoside (Ara-C) and those not treated with drugs were compared. This comparison showed that the sensitivity of myeloblasts to the drug varies from patient to patient, and in one case was different for myeloblasts from bone marrow and from blood from the same patient. We compare the clinical responses of myeloblasts to Ara-C in 6 patients, who had high peripheral blood myeloblast counts, with the sensitivities of their myeloblasts to Ara-C in mice. This comparison indicates that the assay might be a useful way of predicting the response of leukaemic cells in patients to cytotoxic agents. |
format | Text |
id | pubmed-2025424 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1977 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-20254242009-09-10 Assessment of drug sensitivity of human leukaemic myeloblasts. II. The toxic effects of cytosine arabinoside on 125IUdR-labelled human leukaemic myeloblasts in mice. Sonis, S. T. Falcão, R. MacLennan, I. C. Br J Cancer Research Article Leukaemia cells from the peripheral blood and bone marrow of patients with acute myeloblastic leukaemia were labelled in vitro with [125I]5-iodo-2'-deoxy-uridine (IUdR). The myeloblasts were then injected into groups of mice and the survival of these cells estimated by measuring isotope loss, using whole-body counting. The isotope excretion from mice treated with various doses of cytosine arabinoside (Ara-C) and those not treated with drugs were compared. This comparison showed that the sensitivity of myeloblasts to the drug varies from patient to patient, and in one case was different for myeloblasts from bone marrow and from blood from the same patient. We compare the clinical responses of myeloblasts to Ara-C in 6 patients, who had high peripheral blood myeloblast counts, with the sensitivities of their myeloblasts to Ara-C in mice. This comparison indicates that the assay might be a useful way of predicting the response of leukaemic cells in patients to cytotoxic agents. Nature Publishing Group 1977-09 /pmc/articles/PMC2025424/ /pubmed/270373 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Sonis, S. T. Falcão, R. MacLennan, I. C. Assessment of drug sensitivity of human leukaemic myeloblasts. II. The toxic effects of cytosine arabinoside on 125IUdR-labelled human leukaemic myeloblasts in mice. |
title | Assessment of drug sensitivity of human leukaemic myeloblasts. II. The toxic effects of cytosine arabinoside on 125IUdR-labelled human leukaemic myeloblasts in mice. |
title_full | Assessment of drug sensitivity of human leukaemic myeloblasts. II. The toxic effects of cytosine arabinoside on 125IUdR-labelled human leukaemic myeloblasts in mice. |
title_fullStr | Assessment of drug sensitivity of human leukaemic myeloblasts. II. The toxic effects of cytosine arabinoside on 125IUdR-labelled human leukaemic myeloblasts in mice. |
title_full_unstemmed | Assessment of drug sensitivity of human leukaemic myeloblasts. II. The toxic effects of cytosine arabinoside on 125IUdR-labelled human leukaemic myeloblasts in mice. |
title_short | Assessment of drug sensitivity of human leukaemic myeloblasts. II. The toxic effects of cytosine arabinoside on 125IUdR-labelled human leukaemic myeloblasts in mice. |
title_sort | assessment of drug sensitivity of human leukaemic myeloblasts. ii. the toxic effects of cytosine arabinoside on 125iudr-labelled human leukaemic myeloblasts in mice. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025424/ https://www.ncbi.nlm.nih.gov/pubmed/270373 |
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