Changes in sensitivity to radiation and ICRF 159 during the life of monolayer cultures of EMT6 tumour line.

The response of EMT6 mouse tumour cells to ICRF 159, both with and without X-radiation, has been measured during the life of monolayer cultures. The cytotoxic effect of ICRF 159 was found to be proliferation-dependent. Flow cytofluorimetry studies of cell cycle distribution showed that ICRF 159 prev...

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Autores principales: Taylor, I. W., Bleehen, N. M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1977
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025490/
https://www.ncbi.nlm.nih.gov/pubmed/861147
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author Taylor, I. W.
Bleehen, N. M.
author_facet Taylor, I. W.
Bleehen, N. M.
author_sort Taylor, I. W.
collection PubMed
description The response of EMT6 mouse tumour cells to ICRF 159, both with and without X-radiation, has been measured during the life of monolayer cultures. The cytotoxic effect of ICRF 159 was found to be proliferation-dependent. Flow cytofluorimetry studies of cell cycle distribution showed that ICRF 159 prevented cell division while allowing DNA synthesis to continue. This anti-mitotic action and the cytotoxic effect of the drug were found to be closely related. Increased sensitivity to X-radiation was observed in cultures pretreated for 24 h with 200 microgram/ml ICRF 159 In exponential and early plateau cultures this was seen as a reduced shoulder of the survival curve. In late plateau cultures there was no apparent reduction of the shoulder, but an increase in slope.
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spelling pubmed-20254902009-09-10 Changes in sensitivity to radiation and ICRF 159 during the life of monolayer cultures of EMT6 tumour line. Taylor, I. W. Bleehen, N. M. Br J Cancer Research Article The response of EMT6 mouse tumour cells to ICRF 159, both with and without X-radiation, has been measured during the life of monolayer cultures. The cytotoxic effect of ICRF 159 was found to be proliferation-dependent. Flow cytofluorimetry studies of cell cycle distribution showed that ICRF 159 prevented cell division while allowing DNA synthesis to continue. This anti-mitotic action and the cytotoxic effect of the drug were found to be closely related. Increased sensitivity to X-radiation was observed in cultures pretreated for 24 h with 200 microgram/ml ICRF 159 In exponential and early plateau cultures this was seen as a reduced shoulder of the survival curve. In late plateau cultures there was no apparent reduction of the shoulder, but an increase in slope. Nature Publishing Group 1977-05 /pmc/articles/PMC2025490/ /pubmed/861147 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Taylor, I. W.
Bleehen, N. M.
Changes in sensitivity to radiation and ICRF 159 during the life of monolayer cultures of EMT6 tumour line.
title Changes in sensitivity to radiation and ICRF 159 during the life of monolayer cultures of EMT6 tumour line.
title_full Changes in sensitivity to radiation and ICRF 159 during the life of monolayer cultures of EMT6 tumour line.
title_fullStr Changes in sensitivity to radiation and ICRF 159 during the life of monolayer cultures of EMT6 tumour line.
title_full_unstemmed Changes in sensitivity to radiation and ICRF 159 during the life of monolayer cultures of EMT6 tumour line.
title_short Changes in sensitivity to radiation and ICRF 159 during the life of monolayer cultures of EMT6 tumour line.
title_sort changes in sensitivity to radiation and icrf 159 during the life of monolayer cultures of emt6 tumour line.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025490/
https://www.ncbi.nlm.nih.gov/pubmed/861147
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