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Experimental transmission of atypical scrapie to sheep

BACKGROUND: Active surveillance for transmissible spongiform encephalopathies in small ruminants has been an EU regulatory requirement since 2002. A number of European countries have subsequently reported cases of atypical scrapie, similar to previously published cases from Norway, which have pathol...

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Autores principales: Simmons, Marion M, Konold, Timm, Simmons, Hugh A, Spencer, Yvonne I, Lockey, Richard, Spiropoulos, John, Everitt, Sharon, Clifford, Derek
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025597/
https://www.ncbi.nlm.nih.gov/pubmed/17725818
http://dx.doi.org/10.1186/1746-6148-3-20
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author Simmons, Marion M
Konold, Timm
Simmons, Hugh A
Spencer, Yvonne I
Lockey, Richard
Spiropoulos, John
Everitt, Sharon
Clifford, Derek
author_facet Simmons, Marion M
Konold, Timm
Simmons, Hugh A
Spencer, Yvonne I
Lockey, Richard
Spiropoulos, John
Everitt, Sharon
Clifford, Derek
author_sort Simmons, Marion M
collection PubMed
description BACKGROUND: Active surveillance for transmissible spongiform encephalopathies in small ruminants has been an EU regulatory requirement since 2002. A number of European countries have subsequently reported cases of atypical scrapie, similar to previously published cases from Norway, which have pathological and molecular features distinct from classical scrapie. Most cases have occurred singly in flocks, associated with genotypes considered to be more resistant to classical disease. Experimental transmissibility of such isolates has been reported in certain ovinised transgenic mice, but has not previously been reported in the natural host. Information on the transmissibility of this agent is vital to ensuring that disease control measures are effective and proportionate. RESULTS: This report presents the successful experimental transmission, in 378 days, of atypical scrapie to a recipient sheep of homologous genotype with preservation of the pathological and molecular characteristics of the donor. This isolate also transmitted to ovinised transgenic mice (Tg338) with a murine phenotype indistinguishable from that of Nor 98. CONCLUSION: This result strengthens the opinion that these cases result from a distinct strain of scrapie agent, which is potentially transmissible in the natural host under field conditions.
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spelling pubmed-20255972007-10-16 Experimental transmission of atypical scrapie to sheep Simmons, Marion M Konold, Timm Simmons, Hugh A Spencer, Yvonne I Lockey, Richard Spiropoulos, John Everitt, Sharon Clifford, Derek BMC Vet Res Research Article BACKGROUND: Active surveillance for transmissible spongiform encephalopathies in small ruminants has been an EU regulatory requirement since 2002. A number of European countries have subsequently reported cases of atypical scrapie, similar to previously published cases from Norway, which have pathological and molecular features distinct from classical scrapie. Most cases have occurred singly in flocks, associated with genotypes considered to be more resistant to classical disease. Experimental transmissibility of such isolates has been reported in certain ovinised transgenic mice, but has not previously been reported in the natural host. Information on the transmissibility of this agent is vital to ensuring that disease control measures are effective and proportionate. RESULTS: This report presents the successful experimental transmission, in 378 days, of atypical scrapie to a recipient sheep of homologous genotype with preservation of the pathological and molecular characteristics of the donor. This isolate also transmitted to ovinised transgenic mice (Tg338) with a murine phenotype indistinguishable from that of Nor 98. CONCLUSION: This result strengthens the opinion that these cases result from a distinct strain of scrapie agent, which is potentially transmissible in the natural host under field conditions. BioMed Central 2007-08-28 /pmc/articles/PMC2025597/ /pubmed/17725818 http://dx.doi.org/10.1186/1746-6148-3-20 Text en Copyright © 2007 Crown copyright; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Simmons, Marion M
Konold, Timm
Simmons, Hugh A
Spencer, Yvonne I
Lockey, Richard
Spiropoulos, John
Everitt, Sharon
Clifford, Derek
Experimental transmission of atypical scrapie to sheep
title Experimental transmission of atypical scrapie to sheep
title_full Experimental transmission of atypical scrapie to sheep
title_fullStr Experimental transmission of atypical scrapie to sheep
title_full_unstemmed Experimental transmission of atypical scrapie to sheep
title_short Experimental transmission of atypical scrapie to sheep
title_sort experimental transmission of atypical scrapie to sheep
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025597/
https://www.ncbi.nlm.nih.gov/pubmed/17725818
http://dx.doi.org/10.1186/1746-6148-3-20
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